Structure of PDB 7nhn Chain 5 Binding Site BS01

Receptor Information

>7nhn Chain 5 (length=53) Species: 169963 (Listeria monocytogenes EGD-e)

AVPFRRTSKAKKRKRRTHVKLQLPGMNECSNCGEYRLSHHVCPECGQYDG
KDV

Ligand information

>7nhn Chain A (length=2908)

uuaaguuagaaagggcgcacgguggaugccuuggcacuaggagccgaaga
aggacgggacuaacaccgauaugcuuuggggagcuguacguaagcguuga
uccagagauuuccgaaugggggaacccacuaucuuuagucggauaguauc
cuuacgugaauacauagcgugaggaaggcagacccagggaacugaaacau
cuaaguaccuggaggaagagaaagaaaaaucgauuuccugaguagcggcg
agcgaaacggaaagagcccaaaccaagaagcuugcuucuugggguuguag
gacacucuauacggaguuacaaaagaaaguuauaaaugaagcggucugga
aaggcccgccaaagacgguaacagcccgguaguugaaauggcuuucccuc
cagaguggauccugaguacggcggaacacgugaaauuccgucggaauccg
ggaggaccaucucccaaggcuaaauacucccuagugaccgauagugaacc
aguaccgugagggaaaggugaaaagcaccccggaaggggagugaaacagu
uccugaaaccgugugccuacaaguaguuagagcccguuaaugggugauag
cgugccuuuuguagaaugaaccggcgaguuacgauuuguugcaagguuaa
gcggaaaaagcggagccguagcgaaagcgagucugaauagggcgcauaag
uaacaggucguagacccgaaaccaggugaucuacccauguccaggaugaa
gguaagguaauacuuacuggagguccgaacccacgcacguugaaaagugc
ggggaugagguguggguagcggagaaauuccaaucgaacuuggagauagc
ugguucucuccgaaauagcuuuagggcuagccucgagguaaagagucaug
gagguagagcacuguuuggacuaggggcccuucucggguuaccgaauuca
gauaaacuccgaaugccauguacuuauacucgggagucagacugcgagug
auaagauccguagucgaaagggaaacagcccagaccaccaguuaaggucc
ccaaauauauguuaaguggaaaaggaugugggguugcuuagacaaccagg
auguuggcuuagaagcagccaccauugaaagagugcguaauagcucacug
gucgagugaccccgcgccgaaaauguaccggggcuaaacauauuaccgaa
acuguggaugaaccgguucgugguaggagagcguucuaagggcggugaag
ucagaccggaaggacugguggagcgcuuagaagugagaaugccgguauga
guagcgaaagaagggugagaaucccuuccaccgaauaucuaagguuuccu
gaggaaggcucguccgcucaggguuagucgggaccuaagccgaggccgau
aggcguaggcgauggacaacagguagagauuccuguaccagugcuaauug
uuuaaccgauggggugacacagaaggauagggaaucgcacgaauggaaau
gugcguccaagcagugagugugagaaguaggcaaauccgcuucucgcgaa
gcaugagcugugauggggaacggaaguuccugauuucacgcugucaagaa
aagccucuaggaagaguaguacugcccguaccgcaaaccgacacagguag
augaggagagaauccuaaggugagcgagagaacucucguuaaggaacucg
gcaaaaugaccccguaacuucgggagaaggggugcucuauuagggugcaa
gcccgagagagccgcagugaauaggcccaggcgacuguuuagcaaaaaca
caggucucugcaaaaccguaaggugacguauaggggcugacgccugcccg
gugcuggaagguuaagaggagugcuuagcuucggcgaagguacgaauuga
agccccaguaaacggcggccguaacuauaacgguccuaagguagcgaaau
uccuugucggguaaguuccgacccgcacgaaaggcgcaacgaucugggca
cugucucaacgagagacucggugaaauuauaguaccugugaagaugcagg
uuacccgcgacaggacggaaagaccccguggagcuuuacugcaaccugau
auggaauguuuguaccgcuuguacaggauagguaggagccgaagagacgu
gugcgcuagcauacgaggaggcaauggugggauacuacccuggcuguaug
accauucuaacccgccacgcuuagcgcguggggagacagugucagguggg
caguuugacuggggcggucgccuccuaaagaguaacggaggcgcccaaag
guucccucagaauggauggaaaucauucgcagaguguaaaggcacaaggg
agcuugacugcgagacugacaagucgagcagggacgaaagucgggcuuag
ugauccggugguuccgcauggaagggccaucgcucaacggauaaaagcua
ccccggggauaacaggcuuaucucccccaagaguccacaucgacggggag
guuuggcaccucgaugucggcucgucgcauccuggggcuguagucggucc
caaggguugggcuguucgcccauuaaagcggcacgcgagcuggguucaga
acgucgugagacaguucggucccuauccgucgcgggcgcaggaaauuuga
gaggagcuguccuuaguacgagaggaccgggauggacacaccgcuggugu
accaguuguuccgccaggagcaucgcuggguagcuauguguggcagggau
aaacgcugaaagcaucuaagcgugaagccccccucaagaugagauuuccc
auuucuucggaaaguaagaucccugaaagaugaucagguagauagguuug
gaguggaaguguagcgauacauggagcggacaaauacuaaucgaucgagg
acuuaacc

<<<<<<......<<<<<<<<<<<....{[[..[[<<<<<<<[[<<.....
.<<<<<<.((..((.>>>.....<<<<<<<..<<<))..)).>>>.....
>>>>>>>...>>>....<<<....>>><<<<<<<......>>>>>>>.<<
<<<<<<<.........>>>>>>>>>.>>....<<<<<<.....<<....>
>......>>>>>.>.........<<....>>..<<<<<......<<....
.>>.....>>>>>..(((....<<<<<<<<....>>>>>>>>......<<
<<<<<<<.....<<<<........<<<<<<<<<.<<<.....<<<<....
..>>>>.((......<<<.....))>>>...>>>...>>>>>>>>>.>>>
>.>>>>>..>>>>.....<<<<<<<<.........>>>>>>>>......<
<<<<..<<<<>>>>>..)))......]].>>>>>>>.]]...........
....<<....>>....]].........<<<<....>>>>...........
..}....>>>>>>>>>>>.......<<<<..<<<<......>>>>..>>>
>...<.<........>.>.<<<<<<....<<<<<<<<<<<<<..<<<...
<<.......>>...>>>...<<....>>..<<<<.....>>>>......>
>>>>>>>>>>>>..<<.<..<<<<<<<.....<<<<<<<<<..<<<....
<<<<<<......>>>>>>.....>>>...<<<.<<<<<........>>>>
>>>>....>>>>>>>>>...<<<.....>>>......>>>>>>...>..>
.>>..<<<<<<....<<<<<....>>>>><<<<<<<<.....<<<<<<<<
<.....<<<...<<<<<<<<...<<<<<<<.....>>>>>.>>...>>>>
>>>>..>>.>.....>>>>>.>>>>...>>>>>>.>>..<<<<<<<.<<.
.....>>.>>>>>>>....<<<......>>>...<<<<.<<<...<<<((
(.....<<<<<<...........<<..<<<<<<<<<<.......<<<<<.
<<...<<<<.......>>>>...>>.........<<......>>...>>>
>>...>>>>>>>>>>.>>...........))).....>>>>>>.>>>...
>>>>>>><<<<<<..>>>>>>....>>>>>>..<<<<<<<.<<......<
<<<<.<<....>>.>>>>>...>>.>>>>>>>........>>>>>><...
...<<....<<<<<<.......>>>>>><<<<....<<<<<.<<...<<<
<<<...<<.....>>.>>>>>>....((<<<<.<<...<<<...<<<...
.>>>...>>>...>>...<<<<<........>>>>>))<<<<<<<<.<<.
.....<<...<<<<<...<<<<..<<<..<<<<<.<<<<<...(((....
>>>>>.<<..<<<<<..<<<<<<<<<<<.<<.....>>>>>>>>>.....
>>>>..>>>>>...>>..........>>>>>...>>>...>>>>......
..>>>>>..>>..>>.>>>>>>>>>>>>..>>.....<<......>>>>>
>>.<<<<.....>>>>..>>>>..<<<<....<<<<<<<<.<<.......
..<.....<<<<<.....<..>......>>>>><<<<<....<<<<....
>>>>...>>>>>............<<<<<<<<<..<<<.........>>>
..<<<<<<<.....<<<....>>>.......>>>>>>>..<<<<.<<<..
<<<<<<<..<<<...<<...<<<<<..<<<..>>>...>>>>>...>>..
.>>>>>>>>..>>>>><<<<<<.......>>>>>>........<......
.....<<<<<<.....>>>>>>.....>....>>>>...>>.>>>>>>>.
.>..>>.>>>>>>>>.>>>>>>.....>.....<<<<<........>>>>
>..<<<<<<<<.(((.[[......<<<<.[[<<<<..<<<<<.<<<<<<<
<<.<<<<<.<<<<<<.<<<...........<<<<<..<<<.......<<<
<<<<....>>>>>>>....>>>............>>>>>..>>>.>>>>>
>.>>>>>....<<.<<<<<<.....>>>>>>>>.....>>>>>>>>>..>
>>>>..<<<<.....>>>><<<<<<...........>>>>>>..<<...<
<<<<<<...<<<<<<.......>>>>>>.....<<.[.(((>>....>>>
>>>>...<<<<..<<<<....>>>>..>>>>.<<<....>>>.>>)))].
.....<<<<<<<.((..>>>>..))>>>>>>>.............>>>>.
.]]>>>>.......<<<<...<<<<<<...<<.......>>...>>>>>>
....>>>.>.......<<<<<.<<<<<<..<<<<<<<<<......>>>>>
>..>>>.<<<<<.....>>>>>.....>>>>>..>...>>>.>>....<<
<<<<<....>>>.>>>>....]]..)))>>>>>>>>....<<<<<<<...
<<<<..<<<<<<<...............>>>>>>>.<<<<<...<<<<..
.<<<<<<<<<<<.)))>>>>>>..>>>>>...>>>>.>>>>>...<<<..
..<<<<<...........>>>>>....>>>.>>>>.......>>>>>>>.
.<<<<....>>>>....<<<<<<<<<.......>>>>>....<<<<<<<<
..<<....<<<<<....>>>>>...>>...>>>>>.>>>...>>>>....
>>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7nhn Structural basis of ABCF-mediated resistance to pleuromutilin, lincosamide, and streptogramin A antibiotics in Gram-positive pathogens.
• Resolution: 2.9 Å
• Binding residue (original residue number in PDB): A2 V3 P4 F5 R6 R7 T8 S9 K10 A11 K13 R14 K15 R16 R17 T18 K21 N28 R37 S39 H40 Y49 D50
• Binding residue (residue number reindexed from 1): A1 V2 P3 F4 R5 R6 T7 S8 K9 A10 K12 R13 K14 R15 R16 T17 K20 N27 R36 S38 H39 Y48 D49

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0015934 large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7nhn, PDBe:7nhn, PDBj:7nhn
• PDBsum: 7nhn
• PubMed: 34117249
• UniProt: P66207|RL322_LISMO Large ribosomal subunit protein bL32B (Gene Name=rpmF2)