Structure of PDB 8kg8 Chain 4 Binding Site BS01

Receptor Information
>8kg8 Chain 4 (length=697) Species: 559292 (Saccharomyces cerevisiae S288C) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
IWGTNVSIQECTTNFRNFLMSFKYKFRKILDEREEFINNTTDEELYYIKQ
LNEMRELGTSNLNLDARNLLAYKQTEDLYHQLLNYPQEVISIMDQTIKDC
MVSLIVDNNLDYDLDEIETKFYKVRPYNVGSCKGMRELNPNDIDKLINLK
GLVLRSTPVIPDMKVAFFKCNVCDHTMAVEIDRGVIQEPARCERIDCNEP
NSMSLIHNRCSFADKQVIKLQETPDFVPDGQTPHSISLCVYDELVDSCRA
GDRIEVTGTFRSIPIRANSRQRVLKSLYKTYVDVVHVKKVSITDQDLAKI
REVAAREDLYSLLARSIAPSIYELEDVKKGILLQLFGGTNKTFRYRGDIN
ILLCGDPSTSKSQILQYVHKITPRGVYTSGKGSSAVGLTAYITRDVDTKQ
LVLESGALVLSDGGVCCIDEFDKMSDSTRSVLHEVMEQQTISIAKAGIIT
TLNARSSILASANPIGSRYNPNLPVTENIDLPPPLLSRFDLVYLVLDKVD
EKNDRELAKHLTNLYLEDKVLPVEFLTMYISYAKEHIHPIITEAAKTELV
RAYVGMRKMGDEKRITATTRQLESMIRLAEAHAKMKLKNVVELEDVQEAV
RLIRSAIKDYATDPKTGKIDMNLVQTGKSVIQRKLQEDLSREIMNVLKDS
MSFNELIKQINEHSQDRVESSDIQEALSRLQQEDKVIVLGEGVRRSV
Ligand information
Receptor-Ligand Complex Structure
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PDB8kg8 Synergism between CMG helicase and leading strand DNA polymerase at replication fork.
Resolution4.23 Å
Binding residue
(original residue number in PDB)		R449 R451
Binding residue
(residue number reindexed from 1)		R270 R272
Enzymatic activity
Enzyme Commision number 3.6.4.12: DNA helicase.
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0003678 DNA helicase activity
GO:0003688 DNA replication origin binding
GO:0003697 single-stranded DNA binding
GO:0004386 helicase activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0008270 zinc ion binding
GO:0009378 four-way junction helicase activity
GO:0016787 hydrolase activity
GO:0016887 ATP hydrolysis activity
GO:0017116 single-stranded DNA helicase activity
GO:0043138 3'-5' DNA helicase activity
Biological Process
GO:0000727 double-strand break repair via break-induced replication
GO:0006260 DNA replication
GO:0006267 pre-replicative complex assembly involved in nuclear cell cycle DNA replication
GO:0006268 DNA unwinding involved in DNA replication
GO:0006270 DNA replication initiation
GO:0006271 DNA strand elongation involved in DNA replication
GO:0006279 premeiotic DNA replication
GO:0032508 DNA duplex unwinding
GO:0033260 nuclear DNA replication
GO:1902975 mitotic DNA replication initiation
Cellular Component
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005656 nuclear pre-replicative complex
GO:0005737 cytoplasm
GO:0031261 DNA replication preinitiation complex
GO:0031298 replication fork protection complex
GO:0042555 MCM complex
GO:0043596 nuclear replication fork
GO:0071162 CMG complex
GO:0097373 MCM core complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:8kg8, PDBe:8kg8, PDBj:8kg8
PDBsum8kg8
PubMed37730685
UniProtP30665|MCM4_YEAST DNA replication licensing factor MCM4 (Gene Name=MCM4)

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