Structure of PDB 7z20 Chain 4 Binding Site BS01

Receptor Information

>7z20 Chain 4 (length=50) Species: 562 (Escherichia coli)

GIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVRQHVIYKEAK

Ligand information

>7z20 Chain b (length=2901)

gguuaagcgacuaagcguacacgguggaugcccuggcagucagaggcgau
gaaggacgugcuaaucugcgauaagcgucgguaaggugauaugaaccguu
auaaccggcgauuuccgaauggggaaacccaguguguuucgacacacuau
cauuaacugaauccauagguuaaugaggcgaaccgggggaacugaaacau
cuaaguaccccgaggaaaagaaaucaaccgagauucccccaguagcggcg
agcgaacggggagcagcccagagccugaaucaguguguguguuaguggaa
gcgucuggaaaggcgcgcgauacagggugacagccccguacacaaaaaug
cacaugcugugagcucgaugaguagggcgggacacgugguauccugucug
aauauggggggaccauccuccaaggcuaaauacuccugacugaccgauag
ugaaccaguaccgugagggaaaggcgaaaagaaccccggcgaggggagug
aaaaagaaccugaaaccguguacguacaagcagugggagcacgcuuggcg
ugugacugcguaccuuuuguauaaugggucagcgacuuauauucuguagc
aagguuaaccgaauaggggagccgaagggaaaccgagucuuaacugggcg
uuaaguugcaggguauagacccgaaacccggugaucuagccaugggcagg
uugaagguuggguaacacuaacuggaggaccgaaccgacuaauguugaaa
aauuagcggaugacuuguggcugggggugaaaggccaaucaaaccgggag
auagcugguucuccccgaaagcuauuuagguagcgccucgugaauucauc
uccggggguagagcacuguuucggcaagggggucaucccgacuuaccaac
ccgaugcaaacugcgaauaccggagaauguuaucacgggagacacacggc
gggugcuaacguccgucgugaagagggaaacaacccagaccgccagcuaa
ggucccaaagucaugguuaagugggaaacgaugugggaaggcccagacag
ccaggauguuggcuuagaagcagccaucauuuaaagaaagcguaauagcu
cacuggucgagucggccugcgcggaagauguaacggggcuaaaccaugca
ccgaagcugcggcagcgacgcuuaugcguuguuggguaggggagcguucu
guaagccugcgaaggugugcugugaggcaugcuggagguaucagaagugc
gaaugcugacauaaguaacgauaaagcgggugaaaagcccgcucgccgga
agaccaaggguuccuguccaacguuaaucggggcagggugagucgacccc
uaaggcgaggccgaaaggcguagucgaugggaaacagguuaauauuccug
uacuugguguuacugcgaaggggggacggagaaggcuauguuggccgggc
gacgguugucccgguuuaagcguguaggcugguuuuccaggcaaauccgg
aaaaucaaggcugaggcgugaugacgaggcacuacggugcugaagcaaca
aaugcccugcuuccaggaaaagccucuaagcaucagguaacaucaaaucg
uaccccaaaccgacacagguggucagguagagaauaccaaggcgcuugag
agaacucgggugaaggaacuaggcaaaauggugccguaacuucgggagaa
ggcacgcugauauguaggugaagcgacuugcucguggagcugaaaucagu
cgaagauaccagcuggcugcaacuguuuauuaaaaacacagcacugugca
aacacgaaaguggacguauacggugugacgccugcccggugccggaaggu
uaauugaugggguuagcgcaagcgaagcucuugaucgaagccccgguaaa
cggcggccguaacuauaacgguccuaagguagcgaaauuccuugucgggu
aaguuccgaccugcacgaauggcguaaugauggccaggcugucuccaccc
gagacucagugaaauugaacucgcugugaagaugcaguguacccgcggca
agacggaaagaccccgugaaccuuuacuauagcuugacacugaacauuga
gccuugauguguaggauaggugggaggcuuagaaguguggacgccagucu
gcauggagccgaccuugaaauaccacccuuuaauguuugauguucuaacg
uugacccguaauccggguugcggacagugucugguggguaguuugacugg
ggcggucuccuccuaaagaguaacggaggagcacgaagguuggcuaaucc
uggucggacaucaggagguuagugcaauggcauaagccagcuugacugcg
agcgugacggcgcgagcaggugcgaaagcaggucauagugauccgguggu
ucugaauggaagggccaucgcucaacggauaaaagguacuccggggauaa
caggcugauaccgcccaagaguucauaucgacggcgguguuuggcaccuc
gaugucggcucaucacauccuggggcugaaguaggucccaagggugaugc
uguucgccauuuaaagugguacgcgagcuggguuuagaacgucgugagac
aguucggucccuaucugccgugggcgcuggagaacugaggggggcugcuc
cuaguacgagaggaccggaguggacgcaucacugguguucggguugucau
gccaauggcacugcccgguagcuaaaugcggaagagauaagugcugaaag
caucuaagcacgaaacuugccccgagaugaguucucccugacccuuuaag
gguccugaaggaacguugaagacgacgacguugauaggccggguguguaa
gcgcagcgaugcguugagcuaaccgguacuaaugaaccgugaggcuuaac
c

<<<<<<<<......<<<<<<<<<<<.....[[..[[<<<<<<<[[<<...
...<<<<<<.[..(((.>>>.....<<<<<<<..<<<)))..].>>>...
...>>>>>>>...>>>....<<<....>>><<<<<<<....>>>>>>>.<
<<<<<<<<.........>>>>>>>>>.>>...<<<<<<.....<<....>
>......>>>>>.>.........<<....>>..<<<<<<.....<<....
.>>....>>>>>>..(((..<<<<......<<<<<<<<<<<<<.<<<...
<<<<<......>>>>>((......<<<.....))>>>...>>>...>>>>
>>>>>>>>>...>>>>........<<<<<<<<.........>>>>>>>>.
.....<<<<<..<<<<>>>>>..)))......]].>>>>>>>.]].....
..........<<....>>....]].........<<<<.....>>>>....
..............>>>>>>>>>>>.......<<<<..<<<<<<<.>>>>
>>>..>>>>...<.<........>.>.<<<<<<....<<<<<<<<<<<<<
..<<<...<<.....>>...>>>...<<....>>..<<<<.....>>>>.
....>>>>>>>>>>>>>..<<.<...<<<<<<.....<<<<<<<<<..<<
<....<<<<<<......>>>>>>.....>>>...<<<.<<<<<.......
.>>>>>>>>....>>>>>>>>>...<<<.....>>>......>>>>>>..
....>.>>..<<<<<<....<<<<<....>>>>>.<<<<<<<<...<<<<
<<<<<.....<<<...<<<.<<<<...<<................>>...
>>>>.>>>..>>.>.....>>>>>>.>>>...>>>>>>>.>...<<<<<<
<<<<......>>>>>>>>>>....<<<......>>>...<<<<.<<<...
<<<(((.....<<<<<<...........<<..<<<<<..<<........<
<.........<.............>...............<......>..
.....>>....>>..>>>>>.>>...........))).....>>>>>>.>
>>...>>>>>>><<<<<<<........>>>>>>>....>>>>>>..<<<<
<...<<<......<<<<<<<<....>>>>>>>>...>>>..>>>>>....
....>>>>>><......<<.....<<<<<<.......>>>>>><<<<...
.<<<<<.<<...<<<<<<...<<.....>>.>>>>>>....((<<<.<<<
...<<<...<<<....>>>...>>>...>>>..<<<<<........>>>>
>))<<<<<<<<<<<<<...<<<<....<<<<..<<<..<<<<<<<<<<<.
...(((...>>>>>><<..<<<<...<<<<<<<<<<<<.<<.....>>>>
>>>>>>..>>>>...>>>>...>><..<<<<<....>>>>>...>>>>>>
...>>>...>>>>.........>>>>...>>....>>>>>>>>>>>.>>>
..>>.....<<......>>>>>>>.<<<<.....>>>>..>>>>..<<<<
....<<<<<<<<.<<.........<.....<<<<<...............
>>>>><<<<<<.....<<<...<<<<.....>>>>...>>>...>>>>>>
............<<<<<<<<<..<<<.........>>>..<<<<<<<...
..<<<....>>>.......>>>>>>>.<<<<<.<<<..<<<<<<<..<<<
<...<...<<<<<<.<<....>>..>>>>>>..>....>>>>>>>>>..>
>>>><<<<<<.......>>>>>>........<...........<<<<<<.
....>>>>>>.....>....>>>>>..>>.>>>>>>>..>..>>.>>>>>
>>>.>>>>>>.....>.....<<<<<........>>>>>..<<<<<<<<.
(((.[[......<<<<.[[<<<<..<<<<<.<<<.<<<<<.<<<<<<<<<
<<................................................
..................................>>>>>>>>>>>...<<
<.<<<<<<.....>>>>>>>>>....>>>>>.>>>..>>>>>..<<<<..
...>>>><<<<<<...........>>>>>>..<<...<<<<<<<...<<<
<<<.......>>>>>>.....<<...(((>>....>>>>>>>...<<<<.
.<<<<....>>>>..>>>>.<<<....>>>.>>))).......<<<<<<<
.((..>>>>..))>>>>>>>.............>>>>..]]>>>>.....
..<<<<.<.<<<<<<...<<.......>>...>>>>>>...>>>>.>...
....<<<<<.<<<<<<..<<<<<<<<<......>>>>>>..>>>.<..<<
.....>>..>.....>>>>>..>...>>>.>>....<<<<<<<....>>>
.>>>>....]]..)))>>>>>>>>....<<<<<<<...<<<<..<<<<<<
<...............>>>>>>>.<<<<<...<<<<...<<<<<<<<<<<
))).>>>>>>..>>>>>...>>>>.>>>>>...<<<....<<<<<.....
......>>>>>....>>>.>>>>.......>>>>>>>..<<<<<.....>
>>>><....<<..<<<<<.......>>>>>....<<<<<<<<..<<....
<<<<<....>>>>>...>>...>>>>>.>>>.....>>..>..>>>>>>>
>

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7z20 Modulating co-translational protein folding by rational design and ribosome engineering.
• Resolution: 2.29 Å
• Binding residue (original residue number in PDB): R6 K8 F20 T22 T23 K30 L34 K37 F39 R44 H46
• Binding residue (residue number reindexed from 1): R3 K5 F17 T19 T20 K27 L31 K34 F36 R41 H43

Gene Ontology

Molecular Function

• GO:0000049 tRNA binding
• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0000027 ribosomal large subunit assembly
• GO:0002181 cytoplasmic translation
• GO:0006412 translation
• GO:0046677 response to antibiotic

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7z20, PDBe:7z20, PDBj:7z20
• PDBsum: 7z20
• PubMed: 35869078
• UniProt: P0A7N9|RL33_ECOLI Large ribosomal subunit protein bL33 (Gene Name=rpmG)