Structure of PDB 6t83 Chain 4 Binding Site BS01

Receptor Information

>6t83 Chain 4 (length=53) Species: 4932 (Saccharomyces cerevisiae)

ENVWFSHPRRYGKGSRQCRVCSSHTGLIRKYGLNICRQCFREKANDIGFN
KFR

Ligand information

>6t83 Chain a (length=1758)

uaucugguugauccugccaguagucauaugcuugucucaaagauuaagcc
augcaugucuaaguauaagcaauuuauacagugaaacugcgaauggcuca
uuaaaucaguuaucguuuauuugauaguuccuuuacuacaugguauaacu
gugguaauucuagagcuaauacaugcuuaaaaucucgacccuuuggaaga
gauguauuuauuagauaaaaaaucaaugucuucggacucuuugaugauuc
auaauaacuuuucgaaucgcauggccuugugcuggcgaugguucauucaa
auuucugcccuaucaacuuucgaugguaggauaguggccuaccaugguuu
caacggguaacggggaauaaggguucgauuccggagagggagccugagaa
acggcuaccacauccaaggaaggcagcaggcgcgcaaauuacccaauccu
aauucagggagguagugacaauaaauaacgauacagggcccauucggguc
uuguaauuggaaugaguacaauguaaauaccuuaacgaggaacaauugga
gggcaagucuggugccagcagccgcgguaauuccagcuccaauagcguau
auuaaaguuguugcaguuaaaaagcucguaguugaacuuugggcccgguu
ggccggucggauuuccaacggggccuuuccuucuggcuaaccuugagucc
uuguggcucuuggcgaaccaggacuuuuacuuugaaaaaauuagaguguu
caaagcaggcguauugcucgaauauauuagcauggaauaauagaauagga
cguuugguucuauuuuguugguuucuaggaccaucguaaugauuaauagg
gacggucgggggcaucaguauucaauugucagaggugaaauucuuggauu
uauugaagacuaacuacugcgaaagcauuugccaaggacguuuucauuaa
ucaagaacgaaaguuaggggaucgaagaugaucagauaccgucguagucu
uaaccauaaacuaugccgacuagggaucgggugguguuuuuuuaaugacc
cacucggcaccuuacgagaaaucaaagucuuuggguucuggggggaguau
ggucgcaaggcugaaacuuaaaggaauugacggaagggcaccaccaggag
uggagccugcggcuuaauuugacucaacacggggaaacucaccaggucca
gacacaauaaggauugacagauugagagcucuuucuugauuuugugggug
guggugcauggccguucuuaguugguggagugauuugucugcuuaauugc
gauaacgaacgagaccuuaaccuacuaaauaguggugcuagcauuugcug
guuauccacuucuuagagggacuaucgguuucaagccgauggaaguuuga
ggcaauaacaggucugugaugcccuuagacguucugggccgcacgcgcgc
uacacugacggagccagcgagucuaaccuuggccgagaggucuugguaau
cuugugaaacuccgucgugcuggggauagagcauuguaauuauugcucuu
caacgaggaauuccuaguaagcgcaagucaucagcuugcguugauuacgu
cccugcccuuuguacacaccgcccgucgcuaguaccgauugaauggcuua
gugaggccucaggaucugcagagcggagaauuuggacaaacuuggucauu
uagaggaacuaaaagucguaacaagguuuccguaggugaaccugcggaag
gaucauua

...<<<<<...[[[[>>>>>[[[..((((((................[[[
.[[[..<<....<<....<<..........>>...>>.>>......{{..
......[[[..{{..{{....[[[..................<<....<<
.<<.......>>.>>.....>>.........<<<<<..<<....>>..>>
>>>[...((((((......<<<<<<<<<<<<..>>>>.............
.)))))).....]...<<<<..<<<.....>>>.>>>>....]]]...}}
}}..]]].<<<....<<<....<<<<<<<<.......>>>>>>>>>>>..
....>>>...<<<.<<<<....>>>>....>>>.}}.<<.<<<.......
...>>>.>>.<.<<....>>.>...]]]]]].........<<<....<<<
.....>>>..>>>...............<<<<<<<<<<.<......>.>>
>>>>>.>>>......<<..<...........>..>>.........<<<<<
((....<.<<<<.....<<..))>>.......>>>>>>>>>>..))))))
.]]].........[[[{{.......{{...[[[.{{....<<<<<<.<<<
<<....<.>.....>>>>>.>>>>>>....<<<<<<...........<..
.....>...........>>>>>>...<<<<<.<<.......<<...<...
....>..<<<.....>>>....>>......>>.>>..>>>.........[
[[..(((((((.....>>>>>>>>..))))))).]]].....}}....<<
<<<<.<<...<<<<..<<<.<<<<<<.<...<<<......>>>......>
.>>>>>>.>>>.......<<....>>...>>>>..>.>>>>.>>>...]]
]...}}......<<<<<<<...<...<<<<.<.....>.>>>>...>>>>
>>>>..........<<<.<<.<<<..<<<<<<<<.<<<........>>>>
>>>>>>>..>>>...<<..}}>>...>>.....>>>.]]].<<<......
<<<<....>>>>....>>>..]]]]..<<<<<.<<<<<<<..<<.<<<<<
<..<<<.<<<<<<......<..........>..........<<<<<.<..
..<<<<<........<<.<<<........>>>.>>......>>>>>...<
<.<<<..<<<<<<<<<....<<<.<<<<<....>>>...<<<......>>
>...>>.>>>....<<<<<.<<..<<<<..<<<<<.<<<<<<....>>>>
>>...>>>>>..>>>>.>>....<<<<<<.....>>>>>>.......>>>
>>....>>>.>>>...>.>>>>>>>.....>.>>>>>...>>.>>>>.>>
>.....<<<<<<<......<<.....<<..<<<<....>>>>..>>....
>>.......>>>>>>>......<....<<<<<<..........>>>>>>.
...>.....>>>>>>....<<<<<<<<.......>>>>>>>>......>>
...>>>>>>>>>>.>>....<..<<.<..<<<<.<<....<<<<<<<<.<
<<..<.<<..<...<<<<<...>>>>>...>..>>.>..>>>.>>>>>>>
>...>>.>>>>...>.>>...>.....<<<<<<<<<....>>>>>>>>>.
........

Receptor-Ligand Complex Structure

Structure summary

• PDB: 6t83 Molecular mechanism of translational stalling by inhibitory codon combinations and poly(A) tracts.
• Resolution: 4.0 Å
• Binding residue (original residue number in PDB): E4 N5 V6 W7 F8 S9 H10 P11 R12 R13 Y14 G15 K16 G17 S18 R19 C24 T28 G29 L30 I31 R32 K33 Y34 C39 R40 Q41 C42 E45 K54 F55 R56
• Binding residue (residue number reindexed from 1): E1 N2 V3 W4 F5 S6 H7 P8 R9 R10 Y11 G12 K13 G14 S15 R16 C21 T25 G26 L27 I28 R29 K30 Y31 C36 R37 Q38 C39 E42 K51 F52 R53

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0008270 zinc ion binding
• GO:0046872 metal ion binding

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022627 cytosolic small ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:6t83, PDBe:6t83, PDBj:6t83
• PDBsum: 6t83
• PubMed: 31858614
• UniProt: P41057|RS29A_YEAST Small ribosomal subunit protein uS14A (Gene Name=RPS29A)