Structure of PDB 4wt1 Chain 32 Binding Site BS01

Receptor Information

>4wt1 Chain 32 (length=208) Species: 300852 (Thermus thermophilus HB8)

GRYIGPVCRLCRREGVKLYLKGERCYSPKCAMERRPYPPGQHGQKRARRP
SDYAVRLREKQKLRRIYGISERQFRNLFEEASKKKGVTGSVFLGLLESRL
DNVVYRLGFAVSRRQARQLVRHGHITVNGRRVDLPSYRVRPGDEIAVAEK
SRNLELIRQNLEAMKGRKVGPWLSLDVEGMKGKFLRLPDREDLALPVNEQ
LVIEFYSR

Ligand information

>4wt1 Chain 1G (length=1497)

uuggagaguuugauccuggcucagggugaacgcuggcggcgugccuaaga
caugcaagucgugcgggccgcggguccguggucagcggcggacgggugag
uaacgcgugggugaccuacccggaagagggggacaacccggggaaacucg
ggcuaaucccccauguggacccgccccuugggguguguccaaagggcuuu
gcccgcuuccggaugggcccgcgucccaucagcuaguuggugggguaaug
gcccaccaaggcgacgacggguagccggucugagaggauggccggccaca
ggggcacugagacacgggccccacuccuacgggaggcagcaguuaggaau
cuuccgcaaugggcgcaagccugacggagcgacgccgcuuggaggaagaa
gcccuucgggguguaaacuccugaacccgggacgaaacccccgacgaggg
gacugacgguaccgggguaauagcgccggccaacuccgugccagcagccg
cgguaauacggagggcgcgagcguuacccggauucacugggcguaaaggg
cguguaggcggccuggggcgucccaugugaaagaccacggcucaaccgug
ggggagcgugggauacgcucaggcuagacggugggagagggugguggaau
ucccggaguagcggugaaaugcgcagauaccgggaggaacgccgauggcg
aaggcagccaccugguccacccgugacgcugaggcgcgaaagcgugggga
gcaaaccggauuagauacccggguaguccacgcccuaaacgaugcgcgcu
aggucucugggucuccugggggccgaagcuaacgcguuaagcgcgccgcc
uggggaguacggccgcaaggcugaaacucaaaggaauugacgggggcccg
cacaagcgguggagcaugugguuuaauucgaagcaacgcgaagaaccuua
ccaggccuugacaugcuagggaacccgggugaaagccuggggugccccgc
gaggggagcccuagcacaggugcugcauggccgucgucagcucgugccgu
gagguguuggguuaagucccgcaacgagcgcaacccccgccguuaguugc
cagcgguucggccgggcacucuaacgggacugcccgcgaaagcgggagga
aggaggggacgacgucuggucagcauggcccuuacggccugggcgacaca
cgugcuacaaugcccacuacaaagcgaugccacccggcaacggggagcua
aucgcaaaaaggugggcccaguucggauuggggucugcaacccgacccca
ugaagccggaaucgcuaguaaucgcggaucagccaugccgcggugaauac
guucccgggccuuguacacaccgcccgucacgccaugggagcgggcucua
cccgaagucgccgggagccuacgggcaggcgccgaggguagggcccguga
cuggggcgaagucguaacaagguagcuguaccggaaggugcggcugg

.....<<<<..[.((((.>>>>.<<<<.<<<<<..<<<<<<<<..<..<<
<.<<<..<<<..<<.<<<<<<<<..>>>>>>>>.>>>>>......<<...
....<<<<<<<<..<<...<<<<<<<.<<<<<....<<<<<......>>>
>>.....>>>>>...<<<<<.<<<<<....>>>>>.>>>>>..<<<<...
>>>>.>>>>>>>..>>>>>>>>>><<<....<<<..<<<<<<<<......
.>>>>>>>>>>>......>>>..<<<<<<<<....>>>>...>>>>.>>.
<<<<<.<.........>>>>>>.<<<<....>>>>...>>>>>>>.....
...<<<....<<<<....>>>>..>>>..>>.>>>>>>..<<<<......
<<<<....>>>>.....>>>>....<<<<<........<<<<.....>>>
>..........>>>>>......<<<<<(((...<<<<<.....<<.)))>
>.......>>>>>>>>>>..>>>>>>>>>..........<<<((.....<
<<<...<<<.<<<<<<<.<<<<<<<<<<......<<<<<<.....>>>>>
>....>>>>>>>>..>>>>>>>>>...<<<<<<<<...<<<<<<<....<
<<<<<<.<..<<<......>>>.....>.>>>>>>>...........<<.
...>>.>>>>>>>..>>>>>.>>>...>>>...>>>>....<<<<<<...
<<...<<<<.........>>>>...>>>>>>>>..........<<<<<<.
.<<<<<<<<<<...>>>>>>>>>>...<<..))>>.....>>>>>>.>>>
.<<<......<<<<....>>>>....>>>..)))).]..<<<.<<<<<<<
.<<.<<<<<<..<<<<<<<<<<......<<........>>..........
<<<<<<<......<<<<<<<<..<<<<<<......>>>>>>....<<...
...>>...>>>>>>>>..<<.<<<..<<<<<<.......<<<<<<<<<..
..>>>..<<<<......>>>>..>>>>>>.....<<<<.<<<<<<....<
...<<<.....>>>.>.....>>>>>>.....<<<<<....>>>>>....
....>>>>.........>>>...>>>>>>>>....>>>>>>>...>>.>>
>>>>>>.....<<<<<<<.....<<<..<<..<<<<....>>>>..>>..
..>>>.....>>>>>>>......<....<<<<<<<........>>>>>>>
....>.....>>>>>>....<<<<<<<..........>>>>>>>......
>>...>>>>>>>>>>..........<<.<.<<<<.<<<..<<<<<<<<<<
<<<...<.<<<<....<<<....>>>.>>>>.>..>>>>>>>>>>>>>..
>>>.>>>>..>.>>.........<<<<<<<<<....>>>>>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 4wt1 Structural insights into the translational infidelity mechanism.
• Resolution: 3.05 Å
• Binding residue (original residue number in PDB): G2 R3 V8 C9 R10 R13 R14 K22 E24 R25 R35 R36 Y38 P40 G41 Q42 H43 Q45 Y54 A55 L58 R59 Q62 R65 R66 S71 E72 R73 Q74 K84 S113 R115 Q116 R118 Q119 H123 H125 R132 V133 D134 L135 S137 K151 L155 E156 L157 R209
• Binding residue (residue number reindexed from 1): G1 R2 V7 C8 R9 R12 R13 K21 E23 R24 R34 R35 Y37 P39 G40 Q41 H42 Q44 Y53 A54 L57 R58 Q61 R64 R65 S70 E71 R72 Q73 K83 S112 R114 Q115 R117 Q118 H122 H124 R131 V132 D133 L134 S136 K150 L154 E155 L156 R208

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding
• GO:0046872 metal ion binding

Biological Process

• GO:0006412 translation
• GO:0042274 ribosomal small subunit biogenesis

Cellular Component

• GO:0005840 ribosome
• GO:0015935 small ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:4wt1, PDBe:4wt1, PDBj:4wt1
• PDBsum: 4wt1
• PubMed: 26037619
• UniProt: P80373|RS4_THET8 Small ribosomal subunit protein uS4 (Gene Name=rpsD)