Structure of PDB 7nhm Chain 3 Binding Site BS01

Receptor Information

>7nhm Chain 3 (length=56) Species: 93061 (Staphylococcus aureus subsp. aureus NCTC 8325)

KLQITLTRSVIGRPETQRKTVEALGLKKTNSSVVVEDNPAIRGQINKVKH
LVTVEE

Ligand information

>7nhm Chain A (length=2824)

uuaaguuauuaagggcgcacgguggaugccuuggcacuagaagccgauga
aggacguuacuaacgacgauaugcuuuggggagcuguaaguaagcuuuga
uccagagauuuccgaauggggaaacccagcaugaguuaugucauguuauc
gauaugugaauacauagcauaucagaaggcacacccggagaacugaaaca
ucuuaguacccggaggaagagaaagaaaauucgauucccuuaguagcggc
gagcgaaaugggaagagcccaaaccaacaagcuugcuuguugggguugua
ggacacucuauacggaguuacaaaggacgacauuagacgaaucaucugga
aagaugaaucaaagaagguaauaauccuguagucgaaaauguugucucuc
uugaguggauccugaguacgacggagcacgugaaauuccgucggaaucug
ggaggaccaucuccuaaggcuaaauacucucuagugaccgauagugaacc
aguaccgugagggaaaggugaaaagcaccccggaaggggagugaaauaga
accugaaaccgugugcuuacaaguagucagagcccguuaaugggugaugg
cgugccuuuuguagaaugaaccggcgaguuacgauuugaugcaagguuaa
gcaguaaauguggagccguagcgaaagcgagucugaauagggcguuuagu
auuuggucguagacccgaaaccaggugaucuacccuuggucagguugaag
uucagguaacacugaauggaggaccgaaccgacuuacguugaaaagugag
cggaugaacugaggguagcggagaaauuccaaucgaaccuggagauagcu
gguucucuccgaaauagcuuuagggcuagccucaagugaugauuauugga
gguagagcacuguuuggacgaggggccccggguuaccgaauucagacaaa
cuccgaaugccaauuaauuuaacuugggagucagaacaugggugauaagg
uccguguucgaaagggaaacagcccagaccaccagcuaaggucccaaaau
auauguuaaguggaaaaggauguggcguugcccagacaacuaggauguug
gcuuagaagcagccaucauuuaaagagugcguaauagcucacuagucgag
ugacacugcgccgaaaauguaccggggcuaaacauauuaccgaagcugug
gauuguccuuuggacaaugguaggagagcguucuaagggcguugaagcau
gaucguaaggacauguggagcgcuuagaagugagaaugccggugugaguc
gcgaaagacgggugagaaucccguccaccgauugacuaagguuuccagag
gaaggcucguccgcucuggguuagucggguccuaagcugaggccgacagg
cguaggcgauggauaacagguugauauuccuguaccaccuauaaucguuu
uaaucgauggggggacgcaguaggauaggcgaagcgugcgauuggauugc
acgucuaagcaguaaggcugaguauuaggcaaauccgguacucguuaagg
cugagcugugauggggagaagacauugagucuucgagucguugauuucac
acugccgagaaaagccucuagauagaaaauaggugcccguaccgcaaacc
gacacagguagucaagaugagaauucuaaggugagcgagcgaacucucgu
uaaggaacucggcaaaaugaccccguaacuucgggagaaggggugcucuu
uaggguuaacgcccagaagagccgcagugaauaggcccaagcgacuguuu
aucaaaaacacaggucucugcuaaaccguaaggugauguauaggggcuga
cgccugcccggugcuggaagguuaagaggagugguuagcuucugcgaagc
uacgaaucgaagccccaguaaacggcggccguaacuauaacgguccuaag
guagcgaaauuccuugucggguaaguuccgacccgcacgaaaggcguaac
gauuugggcacugucucaacgagagacucggugaaaucauaguaccugug
aagaugcagguuacccgcgacaggacggaaagaccccguggagcuuuacu
guagccugauauugaaauucggcuuucuaacccgcaccacuuaucguggu
gggagacagugucaggcgggcaguuugacuggggcggucgccuccuaaaa
gguaacggaggcgcucaaagguucccucagaaugguuggaaaucauucau
agaguguaaaggcauaagggagcuugacugcgagaccuacaagucgagca
gggucgaaagacggacuuagugauccggugguuccgcauggaagggccau
cgcucaacggauaaaagcuaccccggggauaacaggcuuaucucccccaa
gaguucacaucgacggggagguuuggcaccucgaugucggcucaucgcau
ccuggggcuguagucggucccaaggguugggcuguucgcccauuaaagcg
guacgcgagcuggguucagaacgucgugagacaguucggucccuauccgu
cgugggcguaggaaauuugagaggagcuguccuuaguacgagaggaccgg
gauggacauaccucugguguaccaguugucgugccaacggcauagcuggg
uagcuauguguggacgggauaagugcugaaagcaucuaagcaugaagccc
cccucaagaugagauuucccaacuucgguuauaagaucccucaaagauga
ugagguuaauagguucgagguggaagcauggugacauguggagcugacga
auacuaaucgaucgaagacuuaau

<<<<<<......<<<<<<<<<<<....{[[..[[<<<<<<<[[<<.....
.<<<<<<.((..((.>>>.....<<<<<<<..<<<))..)).>>>.....
>>>>>>>...>>>....<<<....>>><<<<<<<......>>>>>>>.<<
<<<<<<<.........>>>>>>>.>>.>>....<<<<<......<<....
>>.......>>>>.>...[.....<<....>>..<<<<<......<<...
..>>.....>>>>>..(((....<<<<<<<<....>>>>>>>>......<
<<<<<<<<.....<<<<.......<<<<<<<<<<.<<<...<<<<<....
..>>>>>((......<<<.....))>>>...>>>...>>>>>>>>>>>>>
>.>>>>>..>>>>.....<<<<<<<<.........>>>>>>>>......<
<<<<..<<<<>>>>>..)))....].]].>>>>>>>.]]...........
....<<....>>....]].........<<<<....>>>>...........
..}....>>>>>>>>>>>.......<<<<..<<<<......>>>>..>>>
>...<.<........>.>.<<<<<<....<<<<<<<.<<<<<..<<<...
<<<.....>>>...>>>...<<....>>..<<<<.....>>>>.....>>
>>>.>>>>>>>..<<.<...<<<<<<.....<<<<<<<<<..<<<....<
<<<<<......>>>>>>.....>>>...<<<.<<<<<........>>>>>
>>>....>>>>>>>>>...<<<.....>>>......>>>>>>......>.
>>..<<<<<<....<<<<<....>>>>><<<<<<<<<...<<<<<<<<<.
....<<<...<<<<<<<<...<...<<<.>>>...>....>>>>>>>>..
>>.>.....>>>>.>>>>>..>>>>>>>.>>..<<<<<<<<<<......>
>>>>>>>>>....<<<......>>>...<<<<.<<<...<<<(((.....
<<<<<<...........<<..<<<<.<<<<........<<<<<.<<...<
<<<.......>>>>...>>.........<<......>>...>>>>>....
>>>>.>>>>.>>...........))).....>>>>>>.>>>...>>>>>>
><<<<<<<...>>>>>>>....>>>>>><.<<<<<<<.<<......<<<<
<.<<....>>.>>>>>...>>.>>>>>>>..>.....>>>>>><......
<<....<<<<<<.......>>>>>><<<<....<<<<<.<<...<<<<<<
...<<.....>>.>>>>>>....((<<<<.<<.<.<<<...<<<....>>
>...>>>..>>>...<<<<<........>>>>>))<<<<<<<..<<....
..<<<...<<<<....<<<<..<<<..<<<<<.<<<<<<...(((...>>
>>>><<..<<<<<..<<<<<<<<<<..<<.....>>.>>>>>>.....>>
>>..>>>>>...>>...<<<<<<.....>>>>>>...>>>>>...>>>..
.>>>>.........>>>>..>>>.>>..>>>>>>>>>>>..>>.....<<
......>>>>>>>.<<<.......>>>..>>>>..<<<<....<<<<<<<
<.<<.........<.....<<<<<...............>>>>><<<<<<
<.<<<<....>>>>.>>>>>>>............<<<<<<<<<..<<<..
.......>>>..<<<<<<<.....<<<....>>>.......>>>>>>>.<
<<<<.<<<..<<<<<<<..<<<...<<...<<<<<<.<<....>>..>>>
>>>...>>...>>>>>>>>..>>>>><<<<<<.......>>>>>>.....
...<...........<<<<<<.....>>>>>>.....>....>>>>>..>
>.>>>>>>>..>..>>.>>>>>>>>.>>>>>>.....>.....<<<<<..
......>>>>>..<<<<<<<<.(((.[[......<<<<.[[<<<<..<<<
<<.<<<<<<<<<.<<<<.<..>.>>>>....<<.<<<<<<.....>>>>>
>>>.....>>>>>>>>>..>>>>>..<<<<.....>>>><<<<<<.....
......>>>>>>..<<...<<<<<<<...<<<<<<.......>>>>>>..
...<<.[.(((>>....>>>>>>>...<<<<..<<<......>>>..>>>
>.<<<....>>>.>>)))]......<<<<<<<.((..>>>>..))>>>>>
>>.............>>>>..]]>>>>.......<<<<.<.<<<<<<...
<<.......>>...>>>>>>...>>>>.>.......<<<<<.<<<<<<..
<<<<<<<<<......>>>>>>..>>>.<<<<<.....>>>>>.....>>>
>>..>...>>>.>>....<<<<<<<....>>>.>>>>....]]..)))>>
>>>>>>....<<<<<<<...<<<<..<<<<<<<...............>>
>>>>>.<<<<<...<<<<...<<<<<<<<<<<.)))>>>>>>..>>>>>.
..>>>>.>>>>>...<<<....<<<<<...........>>>>>....>>>
.>>>>.......>>>>>>>.<<<....>>>....<<<<<<<<<.......
>>>>>....<<<<<<<<.<<<....<<<<<....>>>>>...>>>..>>>
>>.>>>...>>>>....>>>>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7nhm Structural basis of ABCF-mediated resistance to pleuromutilin, lincosamide, and streptogramin A antibiotics in Gram-positive pathogens.
• Resolution: 3.1 Å
• Binding residue (original residue number in PDB): R10 S11 I13 G14 E17 T22 E24 A25 K30 T31 A42 Q46
• Binding residue (residue number reindexed from 1): R8 S9 I11 G12 E15 T20 E22 A23 K28 T29 A40 Q44

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0015934 large ribosomal subunit
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7nhm, PDBe:7nhm, PDBj:7nhm
• PDBsum: 7nhm
• PubMed: 34117249
• UniProt: P0A0G2|RL30_STAA8 Large ribosomal subunit protein uL30 (Gene Name=rpmD)