Structure of PDB 6gsl Chain 21 Binding Site BS01

Receptor Information

>6gsl Chain 21 (length=203) Species: 300852 (Thermus thermophilus HB8)

MKGILGVKVGMTRIFRDDRAVPVTVILAGPCPVVQRRTPEKDGYTAVQLG
FLPQNPKRVNRPLKGHFAKAGVEPVRILREIRDFNPEGDTVTVEIFKPGE
RVDVTGTSKGRGFAGVMKRWNFAGGPDSHGAHKIHRHPGSIGNRKTPGRV
YKGKKMAGHYGAERVTVMNLEVVDVIPEENLLLVKGAVPGPNGGLVIVRE
TKK

Ligand information

>6gsl Chain 1H (length=2895)

aagaugguaagggcccacgguggaugccucggcacccgagccgaugaagg
acguggcuaccugcgauaagccagggggagccgguagcgggcguggaucc
cuggauguccgaaugggggaacccggccggcgggaacgccggucaccgcg
cuuuuugcgcggggggaaccuggggaacugaaacaucucaguacccagag
gagaggaaagagaaaucgacucccugaguagcggcgagcgaaaggggacc
agccuaaaccguccggcuuguccgggcggggucguggggcccucggacac
cgaauccccagccuagccgaagcuguugggaagcagcgccagagagggug
aaagccccguaggcgaaagguggggggauaggugaggguacccgaguacc
ccgugguucguggagccaugggggaaucugggcggaccaccgccuaaggc
uaaguacuccgggugaccgauagcgcaccaguaccgugagggaaagguga
aaagaaccccgggaggggagugaaauagagccugaaaccgugggcuuaca
agcagucacggccccgcaagggguuguggcgugccuauugaagcaugagc
cggcgacucacggucgugggcgagcuuaagccguugaggcggaggcguag
ggaaaccgaguccgaacagggcgcaaacgggggccgaaaaguccgcggcc
guggacccgaaaccgggcgagcuagcccuggccagggugaagcuggggug
agacccaguggaggcccgaaccggugggggaugcaaaccccucggaugag
cuggggcuaggagugaaaagcuaaccgagcccggagauagcugguucucc
ccgaaaugacuuuagggucagccucaggcgcugacuggggccuguagagc
acugauagggcuagggggcccaccagccuaccaaacccugucaaacuccg
aagggucccagguggagccugggagugagggcgcgagcgauaacguccgc
guccgagcgcgggaacaaccgagaccgccagcuaaggcccccaagucugg
gcuaagugguaaaggauguggcgccgcgaagacagccaggauguuggcuu
agaagcagccaucauuuaaagagugcguaauagcucacuggucgaguggc
gccgcgccgaaaaugaucggggcuuaagcccagcgccgaagcugcggguc
ugggggaugaccccaggcgguaggggagcguucccgaugccgaugaaggc
cgacccgcgagggcggcuggagguaagggaagugcgaaugccggcaugag
uaacgauaaagagggugagaaucccucucgccguaagcccaaggguuccu
acgcaauggucgucagcguaggguuaggcgggaccuaaggugaagccgaa
aggcguagccgaagggcagccgguuaauauuccggcccuucccgcaggug
cgauggggggacgcucuaggcuagggggaccggagccauggacgagcccg
gccagaagcgcagggugggagguaggcaaauccgccucccaacaagcucu
gcgugguggggaagcccguacgggugacaaccccccgaagccagggagcc
aagaaaagccucuaagcacaaccugcgggaacccguaccgcaaaccgaca
caggugggcgggugcaagagcacucaggcgcgcgggagaacccucgccaa
ggaacucugcaaguuggccccguaacuucgggagaaggggugcucccugg
ggugaugagccccggggagccgcagugaacaggcucuggcgacuguuuac
caaaaacacagcucucugcgaacucguaagaggagguauagggagcgacg
cuugcccggugccggaaggucaaggggaggggugcaagccccgaaccgaa
gccccggugaacggcggccguaacuauaacgguccuaagguagcgaaauu
ccuugucggguaaguuccgaccugcacgaaaagcguaacgaccggagcgc
ugucucggcgagggacccggugaaauugaacuggccgugaagaugcggcc
uacccguggcaggacgaaaagaccccguggagcuuuacugcagccuggug
uuggcucuuggucgcgccugcguaggauaggugggagccugugaaccccc
gccuccgggugggggggaggcgccggugaaauaccacccuggcgcggcug
ggggccuaacccucggauggggggacagcgcuuggcgggcaguuugacug
gggcggucgccuccuaaaagguaacggaggcgcccaaagguccccucagg
cgggacggaaauccgccggagagcgcaaggguagaagggggccugacugc
gaggccugcaagccgagcaggggcgaaagccgggccuagugaaccggugg
ucccguguggaagggccaucgaucaacggauaaaaguuaccccggggaua
acaggcugaucucccccgagcguccacagcggcggggagguuuggcaccu
cgaugucggcucgucgcauccuggggcugaagaaggucccaaggguuggg
cuguucgcccauuaaagcggcacgcgagcuggguucagaacgucgugaga
caguucggucucuauccgccacgggcgcaggaggcuugaggggggcucuu
ccuaguacgagaggaccggaagggacgcaccucugguuucccagcugucc
cuccaggggcauaagcuggguagccaugugcggaagggauaaccgcugaa
agcaucuaagcgggaagcccgccccaagaugaggccucccacggcgucaa
gccgguaaggacccgggaagaccacccgguggaugggccggggguguaag
cgccgcgaggcguugagccgaccggucccaaucguccgaggucuu

.<<<......<<<<<<<<<<<.....<<..<<<<<<<<<<<<......<<
<<<<.((..((.>>>.....<<<<<<<..<<<))..)).>>>.....>>>
>>>>...>>>....<<<....>>><<<<<<<<....>>>>>>>>.<<<<<
<.....>>>>>>.>>...<<<<<<.....<......>......>>>>>.>
.........<<....>>...<<<<<.....<<.....>>....>>>>>..
.<<<....<<<<<<<<.....>>>>>>>>......<<<<<<<<<....<<
<...<<<<<.<<<<.<<<...<<<<<......>>>>>((......<<<..
...))>>>...>>>...>>>>..>>>>>..>>>>>>>>>.>>>.....<<
<<<<<<<.......>>>>>>>>>......<<<<<..((((>>>>>..>>>
......>>.>>>>>>.>>...............<<....>>....>>...
......<<<<....>>>>..................>>>>>>>>>>>...
....<<<<<<<<<<<....>>>>>>>>>>>...<............>.<<
<<<<....<<<<<<<<<<<<<..<<<...<<<.....>>>...>>>...<
<....>>..<<<<.....>>>>.....<<.....>>....>>>>>>>>>>
>>>..<<.<...<<<<<<.....<<<<<<<<<..<<<....<<<<<<...
...>>>>>>.....>>>...<<<..<<<<........>>>>.>>>....>
>>>>>>>>...<<<.....>>>......>>>>>>......>.>>..<<<<
<<....<<<<<....>>>>><<<<<<<<<.....<<<<<<<<<...<<<.
..<<<<<<<<.<.<<<<<<......>>>>.>>.>.>>>>>>>>..>>.>.
..>>>>>>>>>.....>>>>>>>.>>..<<<<<<<.<<......>>.>>>
>>>>.....<<<......>>>...<<<<.<<<...<<<(((.....<<<<
<<...........<<..<<<<<<<<<........<<<<<.<....<<<<.
......>>>>.....>........<<......>>...>>>>>....>>>>
>>>>>.>>..........)))......>>>>>>.>>>...>>>>>>><<<
<<<<<.....>>>>>>>>....>>>>>>..<<<<<...<<<......<<<
<<.<<<....>>>>>>>>...>>>..>>>>>........>>>>>><....
..<<.....<<<<<<.......>>>>>><<<<....<<<<<.<<...<<<
<<<...<<.....>>.>>>>>>....((<<<<.<<...<<<...<<<...
.>>>...>>>...>>...<<<<<........>>>>>))<<<<<<<<<<<<
<...<<<<....<<<<<.<<<..<<<<<.<<<<.<<..[[[[...>>>>>
><<<...<<<<<<<<<<<<<<<<.<<.....>>>>>>>>>.....>>>>>
>>>>..>>><...<<<<....>>>>.....>>>>>>...>>>..>>>>>.
........>>>>...>>...>>>>>>>>>>>>>>>..>>.....<<....
..>>>>>>>.<<<<<....>>>>>..>>>>..<<<<....<<<<<<<<.<
<.........<.....<<<<<.....<..>......>>>>><<<<<<<<<
<<<.....>>>>>>>>>>>>............<<<<<<<<<..<<<....
.....>>>..<<<<<<<.....<<<....>>>.......>>>>>>>.<<<
<<.<<<..<<<<<<<..<<<...<<...<<<<<....>>>>>...>>...
>>>>>>>>..>>>>><<<<<<.......>>>>>>........<.......
....<<<<<<.....>>>>>>.....>....>>>>>..>>.>>>>>>>..
>..>>.>>>>>>>>.>>>>>>.....>.....<<<<<........>>>>>
..<<<<<<<<.<<<.........<<<<.<<<<<<..<<<<<.<<<.<<<<
<.<<<<<<.<<<<<<<<<...........<<...<..<<......<<<<<
<<<....>>>>>>>>...>>.............>..>>..>>>>>>>>>.
>>>>>>...<<<<<....>>>>>....>>>>>.>>>..>>>>>..<<<<.
....>>>><<<<<<...........>>>>>>..<<...<<<<<<<...<<
<<<<.......>>>>>>.....<<...(((>>....>>>>>>>...<<<<
..<<<......>>>..>>>>.<<<....>>>.>>))).......<<<<<<
<.<<..))))..>>>>>>>>>.............>>>>..>>>>>>....
...<<<<..<<<<<<<...<<.......>>...>>>>>>>...>>>.>..
.....<<<<<.<<<<<<..<<<<<<<<<......>>>>>>>..>>.<<<<
<.....>>>>>.....>>>>>..>...>>>.>>....<<<<<<<....>>
>.>>>>........>>>>>>>>>>>....<<<<<<<...<<<<..<<<<<
<<...............>>>>>>>.<<<<<...<<<<...<<<<<<<<<<
<]]]]>>>>>>...>>>>>...>>>>.>>>>>...<<<....<<<<<...
........>>>>>....>>>.>>>>.......>>>>>>>..<<.......
..>>....<<<<<<<<<.......>>>>>....<<<<<<<<.<<<....<
<<<<....>>>>>...>>>..>>>>>.>>>...>>>>....>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 6gsl Tautomeric G•U pairs within the molecular ribosomal grip and fidelity of decoding in bacteria.
• Resolution: 3.16 Å
• Binding residue (original residue number in PDB): K8 M11 T12 R13 P22 Q35 R37 K41 D42 Y44 Q48 R58 N60 R61 P62 L63 K64 R76 L78 R79 E80 R82 S108 K109 G110 R111 G112 F113 G115 K118 R119 F122 A123 S128 H129 G130 A131 H132 K133 I134 H135 R136 H137 P138 G139 S140 I141 G142 N143 R144 K145 T146 P147 G148 R149 V150 Y151 K152 K154 M156 A157 H159 Y160 R164 V165 T166 M168 N169 K185 A187 V188 P189 G190 P191 N192 K202 K203
• Binding residue (residue number reindexed from 1): K8 M11 T12 R13 P22 Q35 R37 K41 D42 Y44 Q48 R58 N60 R61 P62 L63 K64 R76 L78 R79 E80 R82 S108 K109 G110 R111 G112 F113 G115 K118 R119 F122 A123 S128 H129 G130 A131 H132 K133 I134 H135 R136 H137 P138 G139 S140 I141 G142 N143 R144 K145 T146 P147 G148 R149 V150 Y151 K152 K154 M156 A157 H159 Y160 R164 V165 T166 M168 N169 K185 A187 V188 P189 G190 P191 N192 K202 K203

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:6gsl, PDBe:6gsl, PDBj:6gsl
• PDBsum: 6gsl
• PubMed: 29931292
• UniProt: Q5SHN8|RL3_THET8 Large ribosomal subunit protein uL3 (Gene Name=rplC)