Structure of PDB 7m4v Chain 2 Binding Site BS01

Receptor Information

>7m4v Chain 2 (length=63) Species: 480119 (Acinetobacter baumannii AB0057)

AKLKTRRGAAKRFKATANGFKRKQAFKRHILTKKSAKRIRQLRGCVMVHV
SDVASVRRMCPYI

Ligand information

>7m4v Chain A (length=2732)

agucaaguaauuaagugcaugugguggaugccuuggcagucagaggcgau
gaaagacgugauagccugcgaaaagcuccggggaggcggcaaauauccuu
ugauccggagaugucugaaugggggaacccaccuacuuuaagguagguau
ugcaacaugaauacauaguguugcaaggcgaacgaggggaagugaaacau
cucaguacccuuaggaaaagaaaucaauugagauucccucaguagcggcg
agcgaacggggaucagcccauuaaguuauguguguuuuaguggaacgcuc
ugggaagugcgaacguagagggugauauucccguacacgaaagggcacac
auaaugaugacgaguagggcgaggcacgugaaaccuugucugaauauggg
gggaccauccuccaaggcuaaauacuccugacugaccgauagugaaccag
uaccgugagggaaaggcgaaaagaaccccugugaggggagugaaauagau
ccugaaaccgcaugcauacaagcagugggagcaccuucguggugugacug
cguaccuuuuguauaaugggucagcgacuuauauucaguagcgagguuaa
ccguauaggggagccguagagaaaucgagucuuaauagggcguuuaguug
cuggguauagacccgaaaccaggcgaucuauccaugagcagguugaaggu
uggguaacacuaacuggaggaccgaacccacugucguugaaaagccaggg
gaugacuuguggauaggggugaaaggcuaaucaagccuggugauagcugg
uucuccccgaaagcuauuuagguagcgccucggacgaauaccauaggggg
uagagcacuguuucggcaccgaugcaaacuccgaauaccuaugaguacua
uccgggagacagacugcgggugcuaacguccguagucaagaggaaaacaa
uccagaccgccagcuaaggccccaaaaucauaguuaagugggaaacgaug
ugggaaggcauagacagcuauagucgagucggccugcgcggaagauguaa
cggggcuaaaacuaugugccgaagcugcggauguauacuuuguauacgug
guaggggagcguucuguaagccgaugaagguguguugagaagcaugcugg
agguaucagaagugcgaaugcugacgugaguaacgacaaaacgggugaaa
aacccguucgccgaaagaccaaggguuccaguccaacguuaaucggggcu
gggugagucgaccccuaaggcgaggccgaaaggcguagucgaugggaaaa
ugguuaauauuccauuacuucuguguaaugcgaugagaggacggagaagg
cuaaaucagccuggcguugguuguccaggugaaaggauguaggcauguau
cuuaggcaaauccgggguacucuaugcugagaucugauagcaagcuguac
uugugcagcgaagugguugaugccaugcuuccaggaaaagucucuaagcu
ucaguuacacaggaaucguacccgaaaccgacacagguggucaggucgag
uagaccaaggcgcuugagagaacucugcugaaggaacuaggcaaaauggu
accguaacuucgggagaagguacgcuguuguuggugauggaacucgcuuc
cugagcugacgacagccgcagaaaccaggccgcugcaacuguuuauuaaa
aacauagcacucugcaaacacgaaaguggacguauagggugugaugccug
cccggugcuggaagguuaauugaugggguuagcguaagcgaagcucuuga
ucgaagccccaguaaacggcggccguaacuauaacgguccuaagguagcg
aaauuccuugucggguaaguuccgaccugcacgaauggcauaaugauggc
ggcgcugucuccagcagaggcucagugaaaucgaaaucgcugugaagaug
caguguacccgcggcuagacggaaagaccccgugaaccuuuacugcagcu
ugacacugaacuuugauugagguucuaacucugucccgugauccgggacg
aggaccgugucugguggguaguuugacuggggcggucuccuccuaaagag
uaacggaggaguacgaaggugcgcucagcguggucggaaaucacgcguag
aguauaaaggcaaaagcgcgcuuaacugcgagacccacaagucgagcagg
uacgaaaguaggucuuagugauccggugguucuguauggaagggccaucg
cucaacggauaaaagguacucuggggauaacaggcugauaccgcccaaga
guucauaucgacggcgguguuuggcaccucgaugucggcucaucucaucc
uggggcugaagcaggucccaaggguauggcuguucgccauuuaaagaggu
acgcgagcuggguuuagaacgucgugagacaguucggucccuaucuaccg
ugggcgcuggaaauuugagaggaucugcuccuaguacgagaggaccagag
uggacgaaccucugguguaccgguugugacgccagucgcaucgccgggua
gcuauguucggaagggauaaccgcugaaagcaucuaagcgggaagccuac
cucaagauaagauuucccuaggacuuuauguccucuaaagagccguucga
gacuaggacguugauagguuggauguggaagcauagugauaugugaagcu
gaccaauacuaauugcucgugaggcuugacua

<<<<<<<<......<<<<<<<<<<<.....[[..[[<<<<<<<[[<<...
...<<<<<<.[..(((.>>>.....<<<<<<<..<<.)))...]..>>..
...>>>>>>>...>>>....<<<....>>><<<<<<<....>>>>>>>.<
<<<<<<<<.........>>>>>>>>>.>>...<<<<<<.....<<....>
>......>>>>>.>.........<<....>>...<<<<<.....<<....
.>>....>>>>>...<<<...<..<<<<<<<<<<<<<<.<<<...<<<.<
......>.>>>((......<<<.....))>>>...>>>...>>>>>>>>>
>>>>>...>.......<<<<<<<<.........>>>>>>>>......<<<
<<..<<<<>>>>>..>>>......]].>>>>>>>.]].............
..<<....>>....]].........<<<<.....>>>>............
......>>>>>>>>>>>.......<<<<..<<<<<.....>>>>>..>>>
>...<.<........>.>.<<<<<<....<<<<<<<<<<<<<..<<<...
<<.....>>...>>>...<<....>>..<<<<.....>>>>.....>>>>
>>>>>>>>>..<<.<..<<<<<<<.....<<<<<<<<<..<<<....<<<
<<<......>>>>>>.....>>>...<<<.<<<.<........>.>>>>>
>....>>>>>>>>>...<<<.....>>>......>>>>>>...>..>.>>
..<<<<<<....<<<<<....>>>>>.<<<<<<<....<<<<<<<<<...
..<<<...<<<.<<<<..>>>>.>>>..>>.>.....>>>>>>.>>>...
>>>>>>.>...<<<<<<<<<<......>>>>>>>>>>....<<<......
>>>...<<<<.<<<...<<<(((.....<<<<<<...........<<..<
<<<<..<<........<<<..>>>....>>..>>>>>.>>..........
.)))......>>>>>>.>>>...>>>>>>><<<<<<<<...>>>>>>>>.
...>>>>>><.<<<<<...<<<......<<<<<<<<....>>>>>>>>..
.>>>..>>>>>..>.....>>>>>><......<<.....<<<<<<.....
..>>>>>><<<<....<<<<<.<<...<<<<<<...<<.....>>.>>>>
>>....((<<<.<<<...<<<...<<<....>>>...>>>...>>>..<<
<<<........>>>>>))<<<<<<<<<<<<<...<<<<....<<<<..<<
<..<<<<<<<<<<<...((((...>>>>>>....<<<<...<<<<.<<<<
<<..<<.....>>.>>>>>>....>>>>...>>>>........<<<<<..
....>>>>>....>>>>>...>>>...>>>>.........>>>>...>>.
...>>>>>>>>>>>.>>>..>>.....<<......>>>>>>>.<<<<...
..>>>>..>>>>..<<<<....<<<<<<<<.<<...............<<
<<<.....<..>......>>>>><<<<<<<<<<<<...<<<<.....>>>
>...>>>>>>>>>>>>............<<<<<<<<<..<<<........
.>>>..<<<<<<<.....<<<....>>>.......>>>>>>>.<<<<<.<
<<..<<<<<<<..<<<<.......<<<<<<.<<....>>..>>>>>>...
....>>>>>>>>>..>>>>><<<<<<.......>>>>>>........<..
.........<<<<<<.....>>>>>>.....>....>>>>>..>>.>>>>
>>>.....>>.>>>>>>>>.>>>>>>.....>.....<<<<<........
>>>>>..<<<<<<<.<<<<.[[......<<<<.[[<<<<..<<<<..<<<
.<<<<<.<<<<<<<<..>>>>>>>>...<<<.<<<<<<.....>>>>>>>
>>....>>>>>.>>>...>>>>..<<<<.....>>>><<<<<<.......
....>>>>>>..<<...<<<<<<<...<<<<<<.......>>>>>>....
.<<.[.(((>>....>>>>>>>...<<<<..<<<......>>>..>>>>.
<<<....>>>.>>)))]......<<<<<<<.((..>>>>..))>>>>>>>
.............>>>>..]]>>>>.......<<<<...<<<<<<...<<
.......>>...>>>>>>....>>>.>.......<<<<<.<<<<<<..<<
<<<<<<<......>>>>>>..>>>.<<<<<.....>>>>>.....>>>>>
..>...>>>.>>....<<<<<<<....>>>.>>>>....]]..>>>>>>>
>>>>....<<<<<<<...<<<<..<<<<<<.................>>>
>>>.<<<<<...<<<<...<<<<<<<<<<<))))>>>>>>..>>>>>...
>>>>.>>>>>...<<<....<<<<<...........>>>>>....>>>.>
>>>.......>>>>>>>..<<<<<.....>>>>><....<<<<<<<<<..
.....>>>>>....<<<<<<<<..<<....<<<<<....>>>>>...>>.
..>>>>>.>>>...>>>>..>..>>>>>>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7m4v Cryo-EM Determination of Eravacycline-Bound Structures of the Ribosome and the Multidrug Efflux Pump AdeJ of Acinetobacter baumannii.
• Resolution: 2.54 Å
• Binding residue (original residue number in PDB): A2 K3 L4 K5 T6 R7 R8 K12 R13 T17 A18 N19 K22 R23 Q25 A26 F27 R29 H30 I31 L32 T33 K34 K38 R39 R41 Q42 R44 H50 R59 Y63
• Binding residue (residue number reindexed from 1): A1 K2 L3 K4 T5 R6 R7 K11 R12 T16 A17 N18 K21 R22 Q24 A25 F26 R28 H29 I30 L31 T32 K33 K37 R38 R40 Q41 R43 H49 R58 Y62

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7m4v, PDBe:7m4v, PDBj:7m4v
• PDBsum: 7m4v
• PubMed: 34044590
• UniProt: B7I693|RL35_ACIB5 Large ribosomal subunit protein bL35 (Gene Name=rpmI)