Structure of PDB 5v8i Chain 1Q Binding Site BS01

Receptor Information

>5v8i Chain 1Q (length=141) Species: 262724 (Thermus thermophilus HB27)

MLMPRRMKYRKQQRGRLKGATKGGDYVAFGDFGLVALEPAWITAQQIEAA
RVAMVRHFRRGGKIFIRIFPDKPYTKKPLEVRMGKGKGNVEGYVAVVKPG
RVMFEVAGVTEEQAMEALRIAGHKLPIKTKIVRRDAYDEAQ

Ligand information

>5v8i Chain 1A (length=2862)

agaugguaagggcccacgguggaugccucggcacccgagccgaugaagga
cguggcuaccugcgauaagccagggggagccgguagcgggcguggauccc
uggauguccgaaugggggaacccggccggcgggaacgccggucaccgcgc
gcgcggggggaaccuggggaacugaaacaucucaguacccagaggagagg
aaagagaaaucgacucccugaguagcggcgagcgaaaggggaccagccua
aaccguccggcuuguccgggcggggucguggggcccucggaccgaauccc
cagccuagccgaagcuguugggaagcagcgccagagagggugaaagcccc
guaggcgaaagguggggggauaggugaggguacccgaguaccccgugguu
cguggagccaugggggaaucugggcggaccaccgccuaaggcuaaguacu
ccgggugaccgauagcgcaccaguaccgugagggaaaggugaaaagaacc
ccgggaggggagugaaauagagccugaaaccgugggcuuacaagcaguca
cggccccgagggguuguggcgugccuauugaagcaugagccggcgacuca
cggucgugggcgagcuuaagccguugaggcggaggcguagggaaaccgag
uccgaacagggcgucuaguccgcggccguggacccgaaaccgggcgagcu
agcccuggccagggugaagcuggggugagacccaguggaggcccgaaccg
gugggggaugcaaaccccucggaugagcuggggcuaggagugaaaagcua
accgagcccggagauagcugguucuccccgaaaugacuuuagggucagcc
ucaggcgcugacuggggccuguagagcacugauagggcuagggggcccac
cagccuaccaaacccugucaaacuccgaagggucccagguggagccuggg
agugagggcgcgagcgauaacguccgcguccgagcgcgggaacaaccgag
accgccagcuaaggcccccaagucugggcuaagugguaaaggauguggcg
ccgcgaagacagccaggagguuggcuuagaagcagccauccuuuaaagag
ugcguaauagcucacuggucgaguggcgccgcgccgaaaaugaucggggc
ucaagcccagcgccgaagcugcgggucugggggaugaccccaggcgguag
gggagcguucccgaugccgaugaaggccgacccgcgagggcggcuggagg
uaagggaagugcgaaugccggcaugaguaacgauaaagagggugagaauc
ccucucgccguaagcccaaggguuccuacgcaauggucgucagcguaggg
uuaggcgggaccuaaggugaagccgaaaggcguagccgaagggcagccgg
uuaauauuccggcccuucccgcaggugcgauggggggacgcucuaggcua
gggggaccggagccauggacgagcccggccagaagcgcagggugggaggu
aggcaaauccgccucccaaaagcucugcgugguggggaagcccgggugac
aaccccccgaagccagggagccaagaaaagccucuaagcacaaccugcgg
gaacccguaccgcaaaccgacacaggugggcgggugcaagagcacucagg
cgcgcgggagaacccucgccaaggaacucugcaaguuggccccguaacuu
cgggagaaggggugcucccuggggugaugagcuccggggagccgcaguga
acaggcucuggcgacuguuuaccaaaaacacagcucucugcgaacucgua
agaggagguauagggagcgacgcuugcccggugccggaaggucaagggga
ggggugcaagccccgaaccgaagccccggugaacggcggccguaacuaua
acgguccuaagguagcgaaauuccuugucggguaaguuccgaccugcacg
aaaagcguaacgaccggagcgcugucucggcgagggacccggugaaauug
aacuggccgugaagaugcggccuacccguggcaggacgaaaagaccccgu
ggagcuuuacugcagccugguguuggcucuuggucgcgccugcguaggau
aggugggagccugugaacccccgccuccgggugggggggaggcgccggug
aaauaccacccuggcgcggcugggggccuaacccucggauggggggacag
cgcuuggcgggcaguuugacuggggcggucgccuccuaaaagguaacgga
ggcgcccaaagguccccucaggcgggacggaaauccgccggagagcgcaa
ggguagaagggggccugacugcgaggccugcaagccgagcaggggcgaaa
gccgggccuagugaaccgguggucccguguggaagggccaucgaucaacg
gauaaaaguuaccccggggauaacaggcugaucucccccgagcguccaca
gcggcggggagguuuggcaccucgaugucggcucgucgcauccuggggcu
gaagaaggucccaaggguugggcuguucgcccauuaaagcggcacgcgag
cuggguucagaacgucgugagacaguucggucucuauccgccacgggcgc
aggaggcuugaggggggcucuuccuaguacgagaggaccggaagggacgc
accucugguuucccagcugucccuccaggggcauaagcuggguagccaug
ugcggaagggauaaccgcugaaagcaucuaagcgggaagcccgccccaag
augaggccucccacggcagccgguaaggacccgggaagacgacccggugg
augggccggggguguaagcgccgcgaggcguugagccgaccggucccaau
cguccgaggucu

<<<......<<<<<<<<<<<.....<<..<<<<<<<<<<<<......<<<
<<<.((..((.>>>.....<<<<<<<..<<<))..)).>>>.....>>>>
>>>...>>>....<<<....>>><<<<<<<<....>>>>>>>>.<<<<<.
.>>>>>.>>...<<<<<<.....<......>......>>>>>.>......
...<<....>>...<<<<<.....<<.....>>....>>>>>...<<<..
..<<<<<<<<.....>>>>>>>>......<<<<<<<<<..<<<...<<<<
<.<<<<.<<<...<<<<<......>>>>>((......<<<.....))>>>
...>>>...>>>>..>>>>>..>>>>>>>>>.>>>.....<<<<<<<<<.
......>>>>>>>>>......<<<<<..((((>>>>>..>>>......>>
.>>>>>>.>>...............<<....>>....>>.........<<
<<....>>>>..................>>>>>>>>>>>.......<<<<
<<<<<<<..>>>>>>>>>>>...<............>.<<<<<<....<<
<<<<<<<<<<<..<<<...<<<.....>>>...>>>...<<....>>..<
<<<.....>>>>.....>>>>>>>>>>>>>..<<.<...<<<<<<.....
<<<<<<<<<..<<<....<<<<<<......>>>>>>.....>>>...<<<
..<<<<........>>>>.>>>....>>>>>>>>>...<<<.....>>>.
.....>>>>>>......>.>>..<<<<<<....<<<<<....>>>>><<<
<<<<<<.....<<<<<<<<<...<<<...<<<<<<<<...<<<<<<....
..>>>>.>>...>>>>>>>>..>>.>...>>>>>>>>>.....>>>>>>>
.>>..<<<<<<<.<<......>>.>>>>>>>.....<<<......>>>..
.<<<<.<<<...<<<(((.....<<<<<<...........<<..<<<<<<
<<<........<<<<<.<<....<<.........>>....>>........
.<<......>>...>>>>>....>>>>>>>>>.>>..........)))..
....>>>>>>.>>>...>>>>>>><<<<<<<<.....>>>>>>>>....>
>>>>>..<<<<<...<<<......<<<<<.<<<....>>>>>>>>...>>
>..>>>>>........>>>>>><......<<.....<<<<<<.......>
>>>>><<<<....<<<<<.<<...<<<<<<...<<.....>>.>>>>>>.
...((<<<<.<<...<<<...<<<....>>>...>>>...>>...<<<<<
........>>>>>))<<<<<<<<<<<<<...<<<<....<<<<<.<<<..
<<<<<.<<<<.<<..[[[[...>>>>>><<<...<<<<<<<<<<<<<<<<
.<<.....>>>>>>>>>....>>>>>>>>>..>>>....<<<..>>>...
...>>>>>...>>>..>>>>>.........>>>>...>>...>>>>>>>>
>>>>>>>..>>.....<<......>>>>>>>.<<<<<....>>>>>..>>
>>..<<<<....<<<<<<<<.<<.........<.....<<<<<.....<.
.>......>>>>><<<<<<<<<<<<.....>>>>>>>>>>>>........
....<<<<<<<<<..<<<.........>>>..<<<<<<<.....<<<...
.>>>.......>>>>>>>.<<<<<.<<<..<<<<<<<..<<<...<<...
<<<<<....>>>>>...>>...>>>>>>>>..>>>>><<<<<<.......
>>>>>>........<...........<<<<<<.....>>>>>>.....>.
...>>>>>..>>.>>>>>>>..>..>>.>>>>>>>>.>>>>>>.....>.
....<<<<<........>>>>>..<<<<<<<<.<<<.........<<<<.
<<<<<<..<<<<<.<<<.<<<<<.<<<<<<<<<<<<<<<<..........
.<<<<<..<<<......<<<<<<<......>>>>>>>...>>>.......
.....>>>>>..>>>>>>>>>>>>>>>>...<<<<<....>>>>>....>
>>>>.>>>..>>>>>..<<<<.....>>>><<<<<<...........>>>
>>>..<<...<<<<<<<...<<<<<<.......>>>>>>.....<<...(
((>>....>>>>>>>...<<<<..<<<......>>>..>>>>.<<<....
>>>.>>))).......<<<<<<<.<<..))))..>>>>>>>>>.......
......>>>>..>>>>>>.......<<<<.<.<<<<<<...<<.......
>>...>>>>>>...>>>>.>.......<<<<<.<<<<<<..<<<<<<<<<
......>>>>>>..>>>.<<<<<.....>>>>>.....>>>>>..>...>
>>.>>....<<<<<<<....>>>.>>>>........>>>>>>>>>>>...
.<<<<<<<...<<<<..<<<<<<<...............>>>>>>>.<<<
<<...<<<<...<<<<<<<<<<<]]]]>>>>>>...>>>>>...>>>>.>
>>>>...<<<....<<<<<...........>>>>>....>>>.>>>>...
....>>>>>>>..<<<<.>>>>....<<<<<<<<<.......>>>>>...
.<<<<<<<<.<<<....<<<<<....>>>>>...>>>..>>>>>.>>>..
.>>>>....>>>

Receptor-Ligand Complex Structure

Structure summary

• PDB: 5v8i Structural robustness of the ribosome inferred from X-ray crystal structures of the 30S ribosomal subunit and the 70S ribosome lacking ribosomal protein uS17
• Resolution: 3.25 Å
• Binding residue (original residue number in PDB): P4 R5 R6 K8 Y9 K11 Q12 Q13 R14 G15 R16 K18 K22 G24 F29 A40 W41 Q46 A49 R56 R59 R60 R67 F69 D71 Y74 K76 K77 E80 R82 M83 G84 K85 G86 K87 R101 R119 H123 K124 P126 K128
• Binding residue (residue number reindexed from 1): P4 R5 R6 K8 Y9 K11 Q12 Q13 R14 G15 R16 K18 K22 G24 F29 A40 W41 Q46 A49 R56 R59 R60 R67 F69 D71 Y74 K76 K77 E80 R82 M83 G84 K85 G86 K87 R101 R119 H123 K124 P126 K128

Gene Ontology

Molecular Function

• GO:0000049 tRNA binding
• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:5v8i, PDBe:5v8i, PDBj:5v8i
• PDBsum: 5v8i
• UniProt: Q72I11|RL16_THET2 Large ribosomal subunit protein uL16 (Gene Name=rplP)