Structure of PDB 7nhl Chain 1 Binding Site BS01

Receptor Information

>7nhl Chain 1 (length=60) Species: 93061 (Staphylococcus aureus subsp. aureus NCTC 8325)

GKQCFVTGRKASTGNRRSHALNSTKRRWNANLQKVRILVDGKPKKVWVSA
RALKSGKVTR

Ligand information

>7nhl Chain A (length=2914)

uuaaguuauuaagggcgcacgguggaugccuuggcacuagaagccgauga
aggacguuacuaacgacgauaugcuuuggggagcuguaaguaagcuuuga
uccagagauuuccgaauggggaaacccagcaugaguuaugucauguuauc
gauaugugaauacauagcauaucagaaggcacacccggagaacugaaaca
ucuuaguacccggaggaagagaaagaaaauucgauucccuuaguagcggc
gagcgaaaugggaagagcccaaaccaacaagcuugcuuguugggguugua
ggacacucuauacggaguuacaaaggacgacauuagacgaaucaucugga
aagaugaaucaaagaagguaauaauccuguagucgaaaauguugucucuc
uugaguggauccugaguacgacggagcacgugaaauuccgucggaaucug
ggaggaccaucuccuaaggcuaaauacucucuagugaccgauagugaacc
aguaccgugagggaaaggugaaaagcaccccggaaggggagugaaauaga
accugaaaccgugugcuuacaaguagucagagcccguuaaugggugaugg
cgugccuuuuguagaaugaaccggcgaguuacgauuugaugcaagguuaa
gcaguaaauguggagccguagcgaaagcgagucugaauagggcguuuagu
auuuggucguagacccgaaaccaggugaucuacccuuggucagguugaag
uucagguaacacugaauggaggaccgaaccgacuuacguugaaaagugag
cggaugaacugaggguagcggagaaauuccaaucgaaccuggagauagcu
gguucucuccgaaauagcuuuagggcuagccucaagugaugauuauugga
gguagagcacuguuuggacgaggggccccggguuaccgaauucagacaaa
cuccgaaugccaauuaauuuaacuugggagucagaacaugggugauaagg
uccguguucgaaagggaaacagcccagaccaccagcuaaggucccaaaau
auauguuaaguggaaaaggauguggcguugcccagacaacuaggauguug
gcuuagaagcagccaucauuuaaagagugcguaauagcucacuagucgag
ugacacugcgccgaaaauguaccggggcuaaacauauuaccgaagcugug
gauuguccuuuggacaaugguaggagagcguucuaagggcguugaagcau
gaucguaaggacauguggagcgcuuagaagugagaaugccggugugaguc
gcgaaagacgggugagaaucccguccaccgauugacuaagguuuccagag
gaaggcucguccgcucuggguuagucggguccuaagcugaggccgacagg
cguaggcgauggauaacagguugauauuccuguaccaccuauaaucguuu
uaaucgauggggggacgcaguaggauaggcgaagcgugcgauuggauugc
acgucuaagcaguaaggcugaguauuaggcaaauccgguacucguuaagg
cugagcugugauggggagaagacauugagucuucgagucguugauuucac
acugccgagaaaagccucuagauagaaaauaggugcccguaccgcaaacc
gacacagguagucaagaugagaauucuaaggugagcgagcgaacucucgu
uaaggaacucggcaaaaugaccccguaacuucgggagaaggggugcucuu
uaggguuaacgcccagaagagccgcagugaauaggcccaagcgacuguuu
aucaaaaacacaggucucugcuaaaccguaaggugauguauaggggcuga
cgccugcccggugcuggaagguuaagaggagugguuagcuucugcgaagc
uacgaaucgaagccccaguaaacggcggccguaacuauaacgguccuaag
guagcgaaauuccuugucggguaaguuccgacccgcacgaaaggcguaac
gauuugggcacugucucaacgagagacucggugaaaucauaguaccugug
aagaugcagguuacccgcgacaggacggaaagaccccguggagcuuuacu
guagccugauauugaaauucggcacagcuuguacaggauagguaggagcc
uuugaaacgugagcgcuagcuuacguggaggcgcuggugggauacuaccc
uagcuguguuggcuuucuaacccgcaccacuuaucguggugggagacagu
gucaggcgggcaguuugacuggggcggucgccuccuaaaagguaacggag
gcgcucaaagguucccucagaaugguuggaaaucauucauagaguguaaa
ggcauaagggagcuugacugcgagaccuacaagucgagcagggucgaaag
acggacuuagugauccggugguuccgcauggaagggccaucgcucaacgg
auaaaagcuaccccggggauaacaggcuuaucucccccaagaguucacau
cgacggggagguuuggcaccucgaugucggcucaucgcauccuggggcug
uagucggucccaaggguugggcuguucgcccauuaaagcgguacgcgagc
uggguucagaacgucgugagacaguucggucccuauccgucgugggcgua
ggaaauuugagaggagcuguccuuaguacgagaggaccgggauggacaua
ccucugguguaccaguugucgugccaacggcauagcuggguagcuaugug
uggacgggauaagugcugaaagcaucuaagcaugaagccccccucaagau
gagauuucccaacuucgguuauaagaucccucaaagaugaugagguuaau
agguucgagguggaagcauggugacauguggagcugacgaauacuaaucg
aucgaagacuuaau

<<<<<<......<<<<<<<<<<<.....[[.(((<<<<<<<[[<<.....
.<<<<<<.((..((.>>>.....<<<<<<<..<<<))..)).>>>.....
>>>>>>>...>>>....<<<....>>><<<<<<<......>>>>>>>.<<
<<<<<<<.........>>>>>>>.>>.>>....<<<<<......<<....
>>.......>>>>.>.........<<....>>..<<<<<......<<...
..>>.....>>>>>..(((....<<<<<<<<....>>>>>>>>......<
<<<<<<<<.....<<<<.......<<<<<<<<<<.<<<...<<<<<....
..>>>>>((......<<<.....))>>>...>>>...>>>>>>>>>>>>>
>.>>>>>..>>>>.....<<<<<<<<.........>>>>>>>>......<
<<<<..<<<<>>>>>..)))......]].>>>>>>>.)))..........
....<<....>>....]].........<<<<....>>>>...........
.......>>>>>>>>>>>.......<<<<..<<<<......>>>>..>>>
>...<.<........>.>.<<<<<<....<<<<<<<.<<<<<..<<<...
<<<.....>>>...>>>...<<....>>..<<<<.....>>>>.....>>
>>>.>>>>>>>..<<.<...<<<<<<.....<<<<<<<<<..<<<....<
<<<<<......>>>>>>.....>>>...<<<.<<<<<........>>>>>
>>>....>>>>>>>>>...<<<.....>>>......>>>>>>......>.
>>..<<<<<<....<<<<<....>>>>><<<<<<<<<...<<<<<<<<<.
....<<<...<<<<<<<<....<.<<<<.>>>>..>....>>>>>>>>..
>>.>.....>>>>.>>>>>..>>>>>>>.>>..<<<<<<<<<<......>
>>>>>>>>>....<<<......>>>...<<<<.<<<...<<<(((.....
<<<<<<...........<<..<<<<.<<<<........<<<<<.<<...<
<<<.......>>>>...>>.........<<......>>...>>>>>....
>>>>.>>>>.>>...........))).....>>>>>>.>>>...>>>>>>
><<<<<<<...>>>>>>>....>>>>>><.<<<<<<<.<<......<<<<
<.<<....>>.>>>>>...>>.>>>>>>>..>.....>>>>>><......
<<....<<<<<<.......>>>>>><<<<....<<<<<.<<...<<<<<<
...<<.....>>.>>>>>>....((<<<<.<<...<<<...<<<....>>
>...>>>...>>...<<<<<........>>>>>))<<<<<<<..<<....
..<<<...<<<<....<<<<..<<<..<<<<<.<<<<<<...(((...>>
>>>><<..<<<<<..<<<<<<<<<<..<<.....>>.>>>>>>.....>>
>>..>>>>>...>>...<<<<<<.....>>>>>>...>>>>>...>>>..
.>>>>.........>>>>..>>>.>>..>>>>>>>>>>>..>>.....<<
......>>>>>>>.<<<.......>>>..>>>>..<<<<....<<<<<<<
<.<<.........<.....<<<<<...............>>>>><<<<<<
<.<<<<....>>>>.>>>>>>>............<<<<<<<<<..<<<..
.......>>>..<<<<<<<.....<<<....>>>.......>>>>>>>.<
<<<<.<<<..<<<<<<<..<<<...<<...<<<<<..<<....>>...>>
>>>...>>...>>>>>>>>..>>>>><<<<<<.......>>>>>>.....
...<...........<<<<<<.....>>>>>>.....>....>>>>>..>
>.>>>>>>>..>..>>.>>>>>>>>.>>>>>>.....>.....<<<<<..
......>>>>>..<<<<<<<<.(((.[[......<<<<.[[<<<<..<<<
<<.<<<<<<<<<.<<<<.<<<<<<<<<<<...........<<<<<..<<<
......<<<<<<<<....>>>>>>>>...>>>............>>>>>.
.>>>>>>>>>>>.>>>>....<<.<<<<<<.....>>>>>>>>.....>>
>>>>>>>..>>>>>..<<<<.....>>>><<<<<<...........>>>>
>>..<<...<<<<<<<...<<<<<<.......>>>>>>.....<<.[.((
(>>....>>>>>>>...<<<<..<<<......>>>..>>>>.<<<....>
>>.>>)))]......<<<<<<<.((..>>>>..))>>>>>>>........
.....>>>>..]]>>>>.......<<<<...<<<<<<...<<.......>
>...>>>>>>....>>>.>.......<<<<<.<<<<<<..<<<<<<<<<.
.....>>>>>>..>>>.<<<<<.....>>>>>.....>>>>>..>...>>
>.>>....<<<<<<<....>>>.>>>>....]]..)))>>>>>>>>....
<<<<<<<...<<<<..<<<<<<<...............>>>>>>>.<<<<
<...<<<<...<<<<<<<<<<<.)))>>>>>>..>>>>>...>>>>.>>>
>>...<<<....<<<<<...........>>>>>....>>>.>>>>.....
..>>>>>>>.<<<....>>>....<<<<<<<<<.......>>>>>....<
<<<<<<<.<<<....<<<<<....>>>>>...>>>..>>>>>.>>>...>
>>>....>>>>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7nhl Structural basis of ABCF-mediated resistance to pleuromutilin, lincosamide, and streptogramin A antibiotics in Gram-positive pathogens.
• Resolution: 3.1 Å
• Binding residue (original residue number in PDB): G2 R10 S13 T14 N16 R18 S19 A21 L22 N23 K26 R27 R28 W29 N30 A31 N32 Q34 K43 K46 A51 R52 K55
• Binding residue (residue number reindexed from 1): G1 R9 S12 T13 N15 R17 S18 A20 L21 N22 K25 R26 R27 W28 N29 A30 N31 Q33 K42 K45 A50 R51 K54

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7nhl, PDBe:7nhl, PDBj:7nhl
• PDBsum: 7nhl
• PubMed: 34117249
• UniProt: Q2FZ60|RL28_STAA8 Large ribosomal subunit protein bL28 (Gene Name=rpmB)