Structure of PDB 3j8g Chain 0 Binding Site BS01

Receptor Information

>3j8g Chain 0 (length=77) Species: 83333 (Escherichia coli K-12)

SRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEKRFVTLRV
SAKGMRVIDKKGIDTVLAELRARGEKY

Ligand information

>3j8g Chain B (length=2874)

gguuaagcgacuaagcguacacgguggaugcccuggcagucagaggcgau
gaaggacgugcuaaucugcgauaagcgucgguaaggugauaugaaccguu
auaaccggcgauuuccgaauggggaaacccaguguguuucgacacacuau
cauuaacugaauccauagguuaaugaggcgaaccgggggaacugaaacau
cuaaguaccccgaggaaaagaaaucaaccgagauucccccaguagcggcg
agcgaacggggagcagcccagagccugaaucaguguguguguuaguggaa
gcgucuggaaaggcgcgcgauacagggugacagccccguacacaaaaaug
cacaugcugugagcucgaugaguagggcgggacacgugguauccugucug
aauauggggggaccauccuccaaggcuaaauacuccugacugaccgauag
ugaaccaguaccgugagggaaaggcgaaaagaaccccggcgaggggagug
aaaaagaaccugaaaccguguacguacaagcagugggagcacgcuuaggc
gugugacugcguaccuuuuguauaaugggucagcgacuuauauucuguag
caagguuaaccgaauaggggagccgaagggaaaccgagucuuaacugggc
guuaaguugcaggguauagacccgaaacccggugaucuagccaugggcag
guugaagguuggguaacacuaacuggaggaccgaaccgacuaauguugaa
aaauuagcggaugacuuguggcugggggugaaaggccaaucaaaccggga
gauagcugguucuccccgaaagcuauuuagguagcgccucgugaauucau
cuccggggguagagcacuguuucggcaagggggucaucccgacuuaccaa
cccgaugcaaacugcgaauaccggagaauguuaucacgggagacacacgg
cgggugcuaacguccgucgugaagagggaaacaacccagaccgccagcua
aggucccaaagucaugguuaagugggaaacgaugugggaaggcccagaca
gccaggauguuggcuuagaagcagccaucauuuaaagaaagcguaauagc
ucacuggucgagucggccugcgcggaagauguaacggggcuaaaccaugc
accgaagcugcggcagcgacgcuuaugcguuguuggguaggggagcguuc
uguaagccugcgaaggugugcugugaggcaugcuggagguaucagaagug
cgaaugcugacauaaguaacgauaaagcgggugaaaagcccgcucgccgg
aagaccaaggguuccuguccaacguuaaucggggcagggugagucgaccc
cuaaggcgaggccgaaaggcguagucgaugggaaacagguuaauauuccu
guacuugguguuacugcgaaggggggacggagaaggcuauguuggccggg
cgacgguugucccgguuuaagcguguaggcugguuuuccaggcaaauccg
gaaaaucaaggcugaggcgugaugacgaggcacuacggugcugaagcaac
aaaugcccugcuuccaggaaaagccucuaagcaucagguaacaucaaauc
guaccccaaaccgacacagguggucagguagagaauaccaaggcgcuuga
gagaacucgggugaaggaacuaggcaaaauggugccguaacuucgggaga
aggcacgcugauauguaggugaggucccucgcggauggagcugaaaucag
ucgaagauaccagcuggcugcaacuguuuauuaaaaacacagcacugugc
aaacacgaaaguggacguauacggugugacgccugcccggugccggaagg
uuaauaucgaagccccgguaaacggcggccguaacuauaacgguccuaag
guagcgaaauuccuugucggguaaguuccgaccugcacgaauggcguaau
gauggccaggcugucuccacccgagacucagugaaauugaacucgcugug
aagaugcaguguacccgcggcaagacggaaagaccccgugaaccuuuacu
auagcuugacacugaacauugagccuugauguguaggauaggugggaggc
uuugaaguguggacgccagucugcauggagccgaccuugaaauaccaccc
uuuaauguuugauguucuaacguugacccguaauccggguugcggacagu
gucugguggguaguuugacuggggcggucuccuccuaaagaguaacggag
gagcacgaagguuggcuaauccuggucggacaucaggagguuagugcaau
ggcauaagccagcuugacugcgagcgugacggcgcgagcaggugcgaaag
caggucauagugauccggugguucugaauggaagggccaucgcucaacgg
auaaaagguacuccggggauaacaggcugauaccgcccaagaguucauau
cgacggcgguguuuggcaccucgaugucggcucaucacauccuggggcug
aaguaggucccaaggguauggcuguucgccauuuaaagugguacgcgagc
uggguuuagaacgucgugagacaguucggucccuaucugccgugggcgcu
ggagaacugaggggggcugcuccuaguacgagaggaccggaguggacgca
ucacugguguucggguugucaugccaauggcacugcccgguagcuaaaug
cggaagagauaagugcugaaagcaucuaagcacgaaacuugccccgagau
gaguucucccugacccuuuaaggguccugaaggaacguugaagacgacga
cguugauaggccggguguguaagcgcagcgaugcguugagcuaaccggua
cuaaugaaccgugaggcuuaaccu

<<<<<<<<......<<<<<<<<<<<.....[[..[[<<<<<<<[[<<...
...<<<<<<.[..(((.>>>.....<<<<<<<..<<<)))..].>>>...
...>>>>>>>...>>>....<<<....>>><<<<<<<....>>>>>>>.<
<<<<<<<<.........>>>>>>>>>.>>...<<<<<<.....<......
>......>>>>>.>.........<<....>>..<<<<<<.....<<....
.>>....>>>>>>..(((..<<<<......<<<<<<<<<<<<<.<<<...
<<<<........>>>>((......<<<.....))>>>...>>>...>>>>
>>>>>>>>>...>>>>........<<<<<<<<.........>>>>>>>>.
.....<<<<<..<<<<>>>>>..)))......]].>>>>>>>.]].....
..........<<....>>....]].........<<<<.....>>>>....
..............>>>>>>>>>>>.......<<<<..<<<<<......>
>>>>..>>>>...<.<........>.>.<<<<<<....<<<<<<<<<<<<
<..<<<...<<.....>>...>>>...<<....>>..<<<<.....>>>>
.....>>>>>>>>>>>>>..<<.<.<.<<<<<<.....<<<<<<<<<..<
<<....<<<<<<......>>>>>>.....>>>...<<<.<<<<<......
..>>>>>>>>....>>>>>>>>>...<<<.....>>>......>>>>>>.
..>..>.>>..<<<<<<....<<<<<....>>>>>.<<<<<<<<...<<<
<<<<<<.....<<<...<<<.<<<<...<<.<<<<.....>>>>..>>..
.>>>>.>>>..>>.>.....>>>>>>.>>>...>>>>>>>.>...<<<<<
<<<<<......>>>>>>>>>>....<<<......>>>...<<<<.<<<..
.<<<(((.....<<<<<<...........<<..<<<<<..<<........
<<<<<.<<...<<<<.......>>>>...>>........<<<......>>
>..>>>>>....>>..>>>>>.>>...........))).....>>>>>>.
>>>...>>>>>>><<<<<<<<<....>>>>>>>>>....>>>>>><<<<<
<<...<<<......<<<<<<<<....>>>>>>>>...>>>..>>>>>.>>
.....>>>>>><<.....<<.....<<<<<<.......>>>>>><<<<..
..<<<<<.<<...<<<<<<...<<.....>>.>>>>>>....((<<<.<<
<...<<<...<<<....>>>...>>>...>>>..<<<<<........>>>
>>))<<<<<<<<<<<<<...<<<<....<<<<..<<<..<<<<<<<<<<<
....(((...>>>>>><<..<<<<...<<<<<<<<<<<<.<<.....>>>
>>>>>>>..>>>>...>>>>...>><..<<<<<....>>>>>...>>>>>
>...>>>...>>>>.........>>>>...>>....>>>>>>>>>>>.>>
>..>>.....<<......>>>>>>>.<<<<.....>>>>..>>>>..<<<
<....<<<<<<<<.<<...............<<<<<....<....>....
.>>>>><<<<<<.....<<<...<<<<.....>>>>...>>>...>>>>>
>............<<<<<<<<<..<<...........>>..<<<<<<<..
...<<<....>>>.......>>>>>>>.<<<<<......<<<<<<...<<
<<........>>>>.>>>>..>>....<<<<<.......>>>>>......
...............<<<<<.......>>>>>..........>>>>>..>
>.>>>>>>>.....>>.>>>>>>>>.>>>>>>...>.>.....<<<<<..
......>>>>>..<<<<<<<<.(((.[[......<<<<.[[<<<<..<<<
<..<<<.<<<<<.<<<<<<<<.<<.<.<......................
..................................................
.>..>.>>.>>>>>>>>...<<<.<<<<<<.....>>>>>>>>>....>>
>>>.>>>...>>>>..<<<<.....>>>><<<<<<...........>>>>
>>..<<...<<<<<<<...<<<<<<.......>>>>>>.....<<...((
(>>....>>>>>>>...<<<<..<<<<....>>>>..>>>>.<<<....>
>>.>>))).......<<<<<<<.((..>>>>..))>>>>>>>........
.....>>>>..]]>>>>.......<<<<...<<<<<<...<<.......>
>...>>>>>>....>>>.>.......<<<<<.<<<<<<..<<<<<<<<<.
.....>>>>>>..>>>.<<<<<.....>>>>>.....>>>>>..>...>>
>.>>....<<<<<<<....>>>.>>>>....]]..)))>>>>>>>>....
<<<<<<<<..<<<<..<<<<<<<.....<....>....>>>>>>>.<<<<
<...<<<<...<<<<<<<<<<<))).>>>>>>..>>>>>...>>>>.>>>
>>...<<<....<<<<<...........>>>>>....>>>.>>>>>....
..>>>>>>>..<<<<<<...>>>>>>.....<<..<<<<.........>>
>>....<<<<<<<<..<<....<<<<<....>>>>>...>>...>>>>>.
>>>.....>>.....>>>>>>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 3j8g Structural insights into the function of a unique tandem GTPase EngA in bacterial ribosome assembly
• Resolution: 5.0 Å
• Binding residue (original residue number in PDB): R2 K9 T13 N15 N16 R17 S18 H19 A20 L21 N22 R27 F28 L29 P30 N31 H35 R36 K53 M55 R56 K60 K61
• Binding residue (residue number reindexed from 1): R2 K9 T13 N15 N16 R17 S18 H19 A20 L21 N22 R27 F28 L29 P30 N31 H35 R36 K53 M55 R56 K60 K61

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0005515 protein binding
• GO:0019843 rRNA binding

Biological Process

• GO:0000027 ribosomal large subunit assembly
• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:3j8g, PDBe:3j8g, PDBj:3j8g
• PDBsum: 3j8g
• PubMed: 25389271
• UniProt: P0A7M2|RL28_ECOLI Large ribosomal subunit protein bL28 (Gene Name=rpmB)